Ashman L K, Ludbrook J, Marshall V R
Clin Chim Acta. 1977 Jan 3;74(1):77-84. doi: 10.1016/0009-8981(77)90390-4.
A direct, paired, comparison was made of two plasma radioimmunoassays for carcinoembryonic antigen (CEA), between a perchloric acid-extracted, ammonium sulphate precipitation method; and a whole-plasma, double-antibody method. For both assays the same preparations of standard CEA, 125I-labelled CEA, and anti-CEA serum were used. Plasma samples were taken from young healthy subjects (24), cancer-free hospital inpatients (44) and cancer-proven untreated patients (94). Within-batch and between-batch variation were both greater for the extracted-plasma method. Cancer discriminatory ability was assessed by probabilistic means. With cancer-free inpatients as the reference group, no difference between the assay methods was revealed. With young healthy subjects as the reference group the whole-plasma assay was superior.
对两种癌胚抗原（CEA）血浆放射免疫测定法进行了直接的配对比较，一种是高氯酸提取、硫酸铵沉淀法，另一种是全血浆双抗体法。两种测定法均使用相同的标准CEA制剂、125I标记的CEA和抗CEA血清。血浆样本取自年轻健康受试者（24例）、无癌住院患者（44例）和经证实未治疗的癌症患者（94例）。提取血浆法的批内和批间变异均更大。通过概率方法评估癌症鉴别能力。以无癌住院患者作为参照组时，未发现测定方法之间存在差异。以年轻健康受试者作为参照组时，全血浆测定法更优。
