Rabilloud T, Carpentier G, Tarroux P
Biochimie et Physiologie du développement CNRS UA686, Paris, France.
Electrophoresis. 1988 Jun;9(6):288-91. doi: 10.1002/elps.1150090608.
High sensitivity and low background, the attractive characteristics of the procedure of Blum et al., Electrophoresis 1987, 8, 93-99, for silver staining of proteins in polyacrylamide gels have been improved by sensitizing the gels with sodium dithionite instead of sodium thiosulfate and by equilibration in water after fixation and prior to sensitization. These modifications decrease the background and allow for a longer development period, which in turn increases sensitivity and color contrast. In addition, the colors of the spots are shifted toward colder tones when compared with the original method.
Blum等人于1987年在《电泳》杂志第8卷第93 - 99页发表的用于聚丙烯酰胺凝胶中蛋白质银染的方法具有高灵敏度和低背景的诱人特性。通过用连二亚硫酸钠而非硫代硫酸钠对凝胶进行敏化处理，并在固定后和敏化前用水平衡，这些特性得到了改善。这些改进降低了背景，并允许更长的显影时间，这反过来又提高了灵敏度和颜色对比度。此外，与原始方法相比，斑点的颜色向冷色调偏移。
