Piralla Antonio, Lunghi Giovanna, Percivalle Elena, Viganò Cristina, Nasta Teresa, Pugni Lorenza, Mosca Fabio, Stronati Mauro, Torresani Erminio, Baldanti Fausto
Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, 27100, Pavia, Italy.
Virology Unit; Fondazione Cà Granda Ospedale Maggiore, Policlinico, Milano, Italy.
Diagn Microbiol Infect Dis. 2014 Jun;79(2):183-6. doi: 10.1016/j.diagmicrobio.2014.02.010. Epub 2014 Feb 24.
FilmArray Respiratory Panel (RP) (Idaho Technology, Inc., Salt Lake City, UT, USA) performance was retrospectively evaluated in respiratory samples collected from neonates in 2 reference neonatology units. Using the FilmArray RP assay, 121/152 (79.6%) samples were positive for at least 1 respiratory virus, while 31/152 (20.4%) were negative. FilmArray RP results were concordant in 68/72 (94.4%) respiratory samples tested with laboratory-developed real-time PCR assays, while in 4/72 (5.6%) samples, the FilmArray RP assay detected an additional virus (2 human rhinovirus/enterovirus and 2 bocavirus). In addition, FilmArray RP results for 70 of 80 (87.5%) respiratory samples tested were concordant with the Seegene Seeplex RV15® detection assay (Seegene, Inc., Seoul, South Korea), while 10/80 (12.5%) were discordant. The advantages of the FilmArray RP are the rapid detection of respiratory viruses (1 hour), the wide number of pathogens detectable in a single assay, and the reduced hands-on time.
对美国犹他州盐湖城爱达荷技术公司的FilmArray呼吸道病原体检测板（RP）在两个新生儿科参考单位收集的新生儿呼吸道样本中的表现进行了回顾性评估。使用FilmArray RP检测，152份样本中有121份（79.6%）至少对1种呼吸道病毒呈阳性，而31份（20.4%）为阴性。在72份用实验室开发的实时PCR检测的呼吸道样本中，FilmArray RP结果与之一致的有68份（94.4%），而在4份（5.6%）样本中，FilmArray RP检测出了另外的病毒（2份人鼻病毒/肠道病毒和2份博卡病毒）。此外，在80份检测的呼吸道样本中，70份（87.5%）的FilmArray RP结果与Seegene Seeplex RV15®检测法（韩国首尔Seegene公司）一致，而10份（12.5%）不一致。FilmArray RP的优点是能快速检测呼吸道病毒（1小时）、单次检测可检测的病原体种类多以及减少了实际操作时间。
