Molecular Virology Laboratory, Department of Laboratory Medicine, University of Washington, Seattle, WA 98102, USA.
Diagn Microbiol Infect Dis. 2012 Dec;74(4):379-83. doi: 10.1016/j.diagmicrobio.2012.08.003. Epub 2012 Sep 13.
The FilmArray Respiratory Panel (Idaho Technology) is a highly multiplexed respiratory virus real-time polymerase chain reaction (PCR) assay. Eighty-four respiratory viruses identified by laboratory-developed real-time reverse transcription-PCR assays (LDA) or by viral cultures were mixed and tested by FilmArray to assess its performance. FilmArray identified 72 (90%) of 80 viruses also detected by LDA. Six of the 8 viruses not detected by FilmArray had PCR cycle threshold values >35. Compared to LDA, FilmArray showed comparable sensitivity when used to test serial dilutions of virus mixtures and good agreement with negative samples. With the use of 1 FilmArray instrument, 7 clinical samples could be analyzed and reported in an 8-h shift compared to 20 using LDA and 1 real-time detection instrument. While the FilmArray was rapid and easy to use, its low throughput and qualitative results may be a disadvantage in some clinical settings.
FilmArray 呼吸道Panel（爱达荷技术公司）是一种高度多重呼吸道病毒实时聚合酶链反应（PCR）检测方法。通过实验室开发的实时逆转录-PCR 检测（LDA）或病毒培养鉴定的 84 种呼吸道病毒进行混合，并通过 FilmArray 进行测试，以评估其性能。FilmArray 鉴定了 80 种病毒中的 72 种（90%），也通过 LDA 检测到。8 种未被 FilmArray 检测到的病毒中，有 6 种 PCR 循环阈值值>35。与 LDA 相比，FilmArray 用于测试病毒混合物的连续稀释液时具有相当的敏感性，与阴性样本的一致性良好。使用 1 台 FilmArray 仪器，与使用 LDA 和 1 台实时检测仪器相比，在 8 小时的轮班中可分析和报告 7 个临床样本。虽然 FilmArray 快速且易于使用，但它的低通量和定性结果在某些临床环境中可能是一个劣势。
