Department of Pathology, St. Jude Children's Research Hospital, Memphis, TN 38105, United States.
J Clin Virol. 2012 Apr;53(4):308-13. doi: 10.1016/j.jcv.2011.12.020. Epub 2012 Jan 31.
The detection of viral respiratory tract infections has evolved greatly with the development of PCR based commercial systems capable of simultaneously detecting a wide variety of pathogens.
Evaluate the relative performance of two commercial broad range systems for the detection of viral agents in clinical respiratory tract specimens from immunocompromised children.
A total of 176 patient samples were included in the analysis, representing only the first sample collected for each patient, and excluding failed reactions. Samples were de-identified and assayed in parallel using two different, broadly multiplexed PCR systems: ResPlex™ II Panel v2.0 (ResPlex), Qiagen, Hilden, Germany and FilmArray(®) Respiratory Panel (FilmArray), Idaho Technology Inc., Salt Lake City, UT. Method comparison was based upon pair-wise concordance of results according to patient age, viral target and number of targets detected.
The two systems showed an overall concordance, by patient, of 83.8% (p=0.0001). FilmArray detected at least one target in 68.8% of samples, while ResPlex detected at least one target in 56.8%. ResPlex failed to detect 20.7% of FilmArray positives, and FilmArray failed to detect 4% of ResPlex positives. The relative performance of each system (including which system detected a higher number of positive samples) varied when stratified by target viral pathogen.
Broadly multiplexed PCR is an effective means of detecting large numbers of clinically relevant respiratory viral pathogens.
随着基于 PCR 的商业系统的发展，能够同时检测多种病原体，病毒呼吸道感染的检测有了很大的发展。
评估两种商业广谱系统在检测免疫功能低下儿童临床呼吸道标本中病毒病原体的相对性能。
共纳入 176 例患者样本进行分析，仅代表每位患者采集的第一份样本，且不包括失败反应。对样本进行去识别，并使用两种不同的广泛多重 PCR 系统平行检测：ResPlex™ II 试剂盒 v2.0（ResPlex），德国希尔德伦的 Qiagen 和 FilmArray（®）呼吸道试剂盒（FilmArray），爱达荷技术公司，盐湖城，犹他州。方法比较基于根据患者年龄、病毒靶标和检测到的靶标数量进行的结果两两一致性。
两种系统的总体一致性为 83.8%（p=0.0001）。FilmArray 检测到至少一个靶标在 68.8%的样本中，而 ResPlex 检测到至少一个靶标在 56.8%的样本中。ResPlex 未能检测到 20.7%的 FilmArray 阳性样本，而 FilmArray 未能检测到 4%的 ResPlex 阳性样本。当按目标病毒病原体分层时，每个系统的相对性能（包括哪个系统检测到更多的阳性样本）有所不同。
广泛多重 PCR 是检测大量临床相关呼吸道病毒病原体的有效方法。
