Qiao Liangjun, Gao Hongwei, Zhang Ting, Jing Lulu, Xiao Chun, Xiao Yue, Luo Ning, Zhu Hongyan, Meng Wentong, Xu Hong, Mo Xianming
1] Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China [2].
Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China.
Sci Rep. 2014 Mar 26;4:4470. doi: 10.1038/srep04470.
The Snail family member snail encodes a zinc finger-containing transcriptional factor that is involved in heart formation. Yet, little is known about how Snail regulates heart development. Here, we identified that one of the duplicated snail genes, snai1b, was expressed in the heart region of zebrafish embryos. Depletion of Snai1b function dramatically reduced expression of α5 integrin, disrupted Fibronectin layer in the heart region, especially at the midline, and prevented migration of cardiac precursors, resulting in defects in cardiac morphology and function in zebrafish embryos. Injection of α5β1 protein rescued the Fibronectin layer and then the myocardial precursor migration in snai1b knockdown embryos. The results provide the molecular mechanism how Snail controls the morphogenesis of heart during embryonic development.
蜗牛家族成员Snail编码一种含锌指的转录因子，该因子参与心脏形成。然而，关于Snail如何调节心脏发育，人们知之甚少。在此，我们发现重复的蜗牛基因之一snai1b在斑马鱼胚胎的心脏区域表达。Snail1b功能的缺失显著降低了α5整合素的表达，破坏了心脏区域特别是中线处的纤连蛋白层，并阻止了心脏前体细胞的迁移，导致斑马鱼胚胎心脏形态和功能出现缺陷。注射α5β1蛋白可挽救snai1b基因敲低胚胎中的纤连蛋白层，进而挽救心肌前体细胞的迁移。这些结果揭示了Snail在胚胎发育过程中控制心脏形态发生的分子机制。
