Wang Jin-Hua, Miao Liang, Li Ming-Yun, Guo Xiao-Fei, Pan Na, Chen Ying-Ying, Zhao Liang
Ningbo University, Ningbo 315211, China.
Dongwuxue Yanjiu. 2014 Mar;35(2):99-107. doi: 10.11813/j.issn.0254-5853.2014.2.099.
The Dmrt family of genes are involved in sex differentiation in different species of invertebrates, and some vertebrates including human. In this study, we cloned the full-length cDNA of ayu (Plecoglossus altivelis) Dmrt1 and DmrtA2. Sequence and phylogenetic tree analyses showed ayu Dmrt1 showed highest similarity to that of Oncorhynchus mykiss while ayu DmrtA2 is most similar to that of Oryzias latipes. Fluorescence-based quantitative reverse transcription PCR (qRT-PCR) revealed the Dmrt1 was predominantly expressed in the testis. At the larval stages, Dmrt1 mRNA expression level was highest during 52-64 days post hatching (dph) and at the gastrula stage during embryonic development. DmrtA2, meanwhile, was specifically expressed in the ovary and was highly expressed in the female brain tissue, but not male brain tissue. During the larval stages, DmrtA2 expression remained high before day 34, and then fluctuated while generally decreasing. During embryonic development, DmrtA2 expression increased gradually and peaked at the hatching stage. Our data suggest that ayu Dmrt1 might participate in the differentiation and maintenance of testis while DmrtA2 may play a role in ovary-differentiation and mature-ovary maintenance. DmrtA2 might also participate in brain development.
Dmrt基因家族参与了不同种类无脊椎动物以及包括人类在内的一些脊椎动物的性别分化过程。在本研究中，我们克隆了香鱼（Plecoglossus altivelis）Dmrt1和DmrtA2的全长cDNA。序列和系统发育树分析表明，香鱼Dmrt1与虹鳟（Oncorhynchus mykiss）的Dmrt1相似度最高，而香鱼DmrtA2与青鳉（Oryzias latipes）的DmrtA2最为相似。基于荧光的定量逆转录PCR（qRT-PCR）显示，Dmrt1主要在睾丸中表达。在幼体阶段，Dmrt1 mRNA表达水平在孵化后52 - 64天（dph）以及胚胎发育的原肠胚阶段最高。与此同时，DmrtA2特异性地在卵巢中表达，并且在雌性脑组织中高表达，而在雄性脑组织中不表达。在幼体阶段，DmrtA2在34天前表达一直很高，然后波动但总体呈下降趋势。在胚胎发育过程中，DmrtA2表达逐渐增加并在孵化阶段达到峰值。我们的数据表明，香鱼Dmrt1可能参与睾丸的分化和维持，而DmrtA2可能在卵巢分化和成熟卵巢维持中发挥作用。DmrtA2也可能参与脑发育。
