Lipitskaia I Ia, Kotkina T I, Tarasov A V, Titov V N
Lab Delo. 1989(2):37-42.
The authors suggest a kinetic method for measuring the blood serum and urine creatinine by Jaffe's test. The method is highly specific, requires little biologic material, and rules out the deproteinization procedure. The specificity of the measurement is effected by selecting the period of time when it is creatinine that reacts with alkaline picrate. A thorough selection of the reaction mixture components and of the order of their addition helps minimize the interference of the substances reacting with alkaline picrate during the same period. The method is adapted to biochemical analyzers.
作者们提出了一种通过杰氏试验测定血清和尿肌酐的动力学方法。该方法具有高度特异性，所需生物材料少，且无需进行脱蛋白程序。通过选择肌酐与碱性苦味酸盐反应的时间段来实现测量的特异性。对反应混合物成分及其添加顺序进行全面选择，有助于将同期与碱性苦味酸盐反应的物质的干扰降至最低。该方法适用于生化分析仪。
