Shanghai Jiao Tong University, School of Biomedical Engineering, No. 800 Dongchuan Road, Shanghai 200240, China.
Peking University, College of Engineering, Department of Biomedical Engineering, Beijing 100871, China.
J Biomed Opt. 2014 Mar;19(3):36019. doi: 10.1117/1.JBO.19.3.036019.
Quantitative analysis of optical clearing effects (OCE) induced by hyperosmotic agents is very important to optical tissue clearing applications in biomedical diagnostic imaging and therapeutics. This study aims at investigating the effect of glycerol concentration on the laser-scanning optical-resolution photoacoustic microscopy (LSOR-PAM) imaging contrast and light penetration depth. The photoacoustic (PA) signal amplitude changes are evaluated as a function of the concentration of glycerol. The results reveal that the PA signal amplitudes are enhanced with the glycerol concentration increasing, and also show that higher concentration of glycerol produces better light penetration and OCE on a phantom. The PA signal amplitude increases only 8.1% for 20% glycerol, but for higher concentrations, the increases are 76% and 165% for 40% and 60% glycerol, respectively. This preliminary study demonstrates that application of glycerol as an optical contrast agent reduces the tissue scattering and is beneficial to PAM imaging and optical diagnosis in clinical dermatology.
高渗剂诱导光透明效应（OCE）的定量分析对于生物医学诊断成像和治疗中的光学组织透明化应用非常重要。本研究旨在研究甘油浓度对激光扫描光分辨率光声显微镜（LSOR-PAM）成像对比度和光穿透深度的影响。评估了光声（PA）信号幅度随甘油浓度的变化。结果表明，随着甘油浓度的增加，PA 信号幅度增强，并且还表明较高浓度的甘油在体模上产生更好的光穿透和 OCE。PA 信号幅度仅增加 20%甘油的 8.1%，但对于更高的浓度，40%和 60%甘油的增加分别为 76%和 165%。这项初步研究表明，甘油作为光学对比剂的应用降低了组织散射，有利于临床皮肤科的 PAM 成像和光学诊断。
