光透明辅助光声显微镜，具有增强的分辨率和成像深度。
Optical clearing-aided photoacoustic microscopy with enhanced resolution and imaging depth.
机构信息
Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, USA.
出版信息
Opt Lett. 2013 Jul 15;38(14):2592-5. doi: 10.1364/OL.38.002592.
Abstract
Due to strong light scattering in tissue, both the spatial resolution and maximum penetration depth of optical-resolution photoacoustic microscopy (OR-PAM) deteriorate sharply with depth. To reduce tissue scattering, we propose to use glycerol as an optical clearing agent in OR-PAM. Our results show that the imaging performance of OR-PAM can be greatly enhanced by optical clearing both in vitro and in vivo.
摘要
由于组织内强烈的光散射，光学分辨率光声显微镜（OR-PAM）的空间分辨率和最大穿透深度都会随深度急剧恶化。为了减少组织散射，我们提出在 OR-PAM 中使用甘油作为光学透明剂。我们的结果表明，光学透明处理可大大增强 OR-PAM 的体外和体内成像性能。
相似文献
1
Optical clearing-aided photoacoustic microscopy with enhanced resolution and imaging depth.光透明辅助光声显微镜，具有增强的分辨率和成像深度。
Opt Lett. 2013 Jul 15;38(14):2592-5. doi: 10.1364/OL.38.002592.
2
Virtual-point-based deconvolution for optical-resolution photoacoustic microscopy.基于虚拟点的光分辨光声显微镜反卷积。
J Biophotonics. 2024 Aug;17(8):e202400078. doi: 10.1002/jbio.202400078. Epub 2024 Jun 27.
3
Optical clearing agents improve photoacoustic imaging in the optical diffusive regime.光学透明剂可改善光学漫射区域的光声成像。
Opt Lett. 2013 Oct 15;38(20):4236-9. doi: 10.1364/OL.38.004236.
4
Concentration dependence of optical clearing on the enhancement of laser-scanning optical-resolution photoacoustic microscopy imaging.浓度依赖性光学清除对激光扫描光分辨光声显微镜成像增强的影响。
J Biomed Opt. 2014 Mar;19(3):36019. doi: 10.1117/1.JBO.19.3.036019.
5
Compressed sensing based virtual-detector photoacoustic microscopy in vivo.基于压缩感知的虚拟探测器光声显微镜活体成像。
J Biomed Opt. 2014 Mar;19(3):36003. doi: 10.1117/1.JBO.19.3.036003.
6
Inverted multiscale optical resolution photoacoustic microscopy.倒置多尺度光学分辨率光声显微镜
J Biophotonics. 2017 Dec;10(12):1580-1585. doi: 10.1002/jbio.201600246. Epub 2017 Jan 27.
7
Microvascular quantification based on contour-scanning photoacoustic microscopy.基于轮廓扫描光声显微镜的微血管定量分析。
J Biomed Opt. 2014 Sep;19(9):96011. doi: 10.1117/1.JBO.19.9.096011.
8
1064 nm acoustic resolution photoacoustic microscopy.1064nm 声学分辨率光声显微镜。
J Biophotonics. 2019 May;12(5):e201800357. doi: 10.1002/jbio.201800357. Epub 2019 Jan 21.
9
Wide-field two-dimensional multifocal optical-resolution photoacoustic-computed microscopy.宽场二维多点光声计算显微镜。
Opt Lett. 2013 Dec 15;38(24):5236-9. doi: 10.1364/OL.38.005236.
10
Super-resolution reconstruction algorithm for optical-resolution photoacoustic microscopy images based on sparsity and deconvolution.基于稀疏性和去卷积的光学分辨率光声显微镜图像超分辨率重建算法
Opt Express. 2023 Jan 2;31(1):598-609. doi: 10.1364/OE.471807.
引用本文的文献
1
Tartrazine-enabled optical clearing for in vivo optical resolution photoacoustic microscopy.用于体内光学分辨率光声显微镜的柠檬黄辅助光学透明化
Biomed Opt Express. 2025 May 29;16(6):2504-2515. doi: 10.1364/BOE.565643. eCollection 2025 Jun 1.
2
Photoacoustic Imaging in Biomedicine and Life Sciences.生物医学与生命科学中的光声成像
Life (Basel). 2022 Apr 14;12(4):588. doi: 10.3390/life12040588.
3
Stokes polarization imaging applied for monitoring dynamic tissue optical clearing.斯托克斯偏振成像技术用于监测动态组织光学透明化。
Biomed Opt Express. 2021 Jul 13;12(8):4821-4836. doi: 10.1364/BOE.426653. eCollection 2021 Aug 1.
4
Chronic cranial window for photoacoustic imaging: a mini review.用于光声成像的慢性颅窗：一篇综述
Vis Comput Ind Biomed Art. 2021 May 26;4(1):15. doi: 10.1186/s42492-021-00081-1.
5
Visualization of skin microvascular dysfunction of type 1 diabetic mice using in vivo skin optical clearing method.利用体内皮肤光学透明化方法观察 1 型糖尿病小鼠皮肤微血管功能障碍。
J Biomed Opt. 2018 Aug;24(3):1-9. doi: 10.1117/1.JBO.24.3.031003.
6
Low Temperature-Mediated Enhancement of Photoacoustic Imaging Depth.低温介导的光声成像深度增强
Sci Rep. 2018 Mar 20;8(1):4873. doi: 10.1038/s41598-018-22898-2.
7
Dictionary learning-based reverberation removal enables depth-resolved photoacoustic microscopy of cortical microvasculature in the mouse brain.基于字典学习的混响消除可实现小鼠大脑皮质微血管的深度分辨光声显微镜成像。
Sci Rep. 2018 Jan 17;8(1):985. doi: 10.1038/s41598-017-18860-3.
8
Motionless volumetric photoacoustic microscopy with spatially invariant resolution.具有空间不变分辨率的静态体积光声显微镜。
Nat Commun. 2017 Oct 3;8(1):780. doi: 10.1038/s41467-017-00856-2.
9
Current status, pitfalls and future directions in the diagnosis and therapy of lymphatic malformation.淋巴管畸形诊断与治疗的现状、陷阱及未来方向
J Biophotonics. 2018 Aug;11(8):e201700124. doi: 10.1002/jbio.201700124. Epub 2017 Nov 5.
10
Enhancement of short coherence digital holographic microscopy by optical clearing.通过光学透明化增强短相干数字全息显微镜
Biomed Opt Express. 2017 Mar 2;8(4):2036-2054. doi: 10.1364/BOE.8.002036. eCollection 2017 Apr 1.
本文引用的文献
1
Recent progress in tissue optical clearing.组织光学透明化的最新进展。
Laser Photon Rev. 2013 Sep;7(5):732-757. doi: 10.1002/lpor.201200056. Epub 2013 Feb 5.
2
Photoacoustic microscopy of bilirubin in tissue phantoms.组织体模中胆红素的光声显微镜成像。
J Biomed Opt. 2012 Dec;17(12):126019. doi: 10.1117/1.JBO.17.12.126019.
3
Switchable skin window induced by optical clearing method for dermal blood flow imaging.光透明化方法诱导的可切换皮肤窗口用于皮肤血流成像。
J Biomed Opt. 2013 Jun;18(6):061209. doi: 10.1117/1.JBO.18.6.061209.
4
Photoacoustic tomography: in vivo imaging from organelles to organs.光声断层成像：从细胞器到器官的活体成像。
Science. 2012 Mar 23;335(6075):1458-62. doi: 10.1126/science.1216210.
5
Photoacoustic tomography: fundamentals, advances and prospects.光声断层成像：原理、进展与展望。
Contrast Media Mol Imaging. 2011 Sep-Oct;6(5):332-45. doi: 10.1002/cmmi.443.
6
Label-free oxygen-metabolic photoacoustic microscopy in vivo.无标记氧代谢光声显微镜活体成像。
J Biomed Opt. 2011 Jul;16(7):076003. doi: 10.1117/1.3594786.
7
Imaging dermal blood flow through the intact rat skin with an optical clearing method.用光学透明化方法对完整大鼠皮肤的真皮血流进行成像。
J Biomed Opt. 2010 Mar-Apr;15(2):026008. doi: 10.1117/1.3369739.
8
In vivo photoacoustic imaging of transverse blood flow by using Doppler broadening of bandwidth.利用带宽的多普勒展宽进行体内横向血流的光声成像。
Opt Lett. 2010 May 1;35(9):1419-21. doi: 10.1364/OL.35.001419.
9
Photoacoustic correlation spectroscopy and its application to low-speed flow measurement.光声相关光谱学及其在低速流量测量中的应用。
Opt Lett. 2010 Apr 15;35(8):1200-2. doi: 10.1364/OL.35.001200.
10
In vivo skin optical clearing by glycerol solutions: mechanism.甘油溶液的体内皮肤光学透明化：机制。
J Biophotonics. 2010 Jan;3(1-2):44-52. doi: 10.1002/jbio.200910080.
Zotero 插件