Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, USA.
Opt Lett. 2013 Jul 15;38(14):2592-5. doi: 10.1364/OL.38.002592.
Due to strong light scattering in tissue, both the spatial resolution and maximum penetration depth of optical-resolution photoacoustic microscopy (OR-PAM) deteriorate sharply with depth. To reduce tissue scattering, we propose to use glycerol as an optical clearing agent in OR-PAM. Our results show that the imaging performance of OR-PAM can be greatly enhanced by optical clearing both in vitro and in vivo.
由于组织内强烈的光散射，光学分辨率光声显微镜（OR-PAM）的空间分辨率和最大穿透深度都会随深度急剧恶化。为了减少组织散射，我们提出在 OR-PAM 中使用甘油作为光学透明剂。我们的结果表明，光学透明处理可大大增强 OR-PAM 的体外和体内成像性能。
