单纯疱疹病毒的生长、制备及检测。
Herpes simplex virus growth, preparation, and assay.
作者信息
Marconi Peggy, Manservigi Roberto
机构信息
Section of Applied Microbiology and Pathology, Department of Life Sciences and Biotechnology (SVeB), University of Ferrara, Via Luigi Borsari 46, Ferrara, 44121, Italy,
出版信息
Methods Mol Biol. 2014;1144:19-29. doi: 10.1007/978-1-4939-0428-0_2.
PMID:24671674
Abstract
In order to study the biology of herpes simplex virus or to use it as a vector in gene therapy, it is necessary to grow the virus and to prepare virus stocks. Many different protocols are available from different research groups working with herpes simplex virus type 1 or 2 (HSV-1 or HSV-2). This chapter describes the procedures used in our laboratory.
摘要
为了研究单纯疱疹病毒的生物学特性或将其用作基因治疗的载体，有必要培养该病毒并制备病毒储备液。许多不同的研究小组针对1型或2型单纯疱疹病毒（HSV-1或HSV-2）提供了多种不同的实验方案。本章介绍了我们实验室所采用的方法。
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