Sawtell Nancy M, Thompson Richard L
Division of Infectious Diseases, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, 3333 Burnet Ave., MLC 7017, Cincinnati, OH, 45229-3039, USA,
Methods Mol Biol. 2014;1144:129-47. doi: 10.1007/978-1-4939-0428-0_9.
Two important components to a useful strategy to examine viral gene regulation in vivo are (1) a highly efficient protocol to generate viral mutants that limits undesired mutation and retains full replication competency in vivo and (2) an efficient system to detect and quantify viral promoter activity in rare cells in vivo. Our strategy and protocols for generating, characterizing, and employing HSV viral promoter/reporter mutants in vivo are provided in this two-part chapter.
（1）一种高效的方案，用于产生病毒突变体，该方案可限制不必要的突变并在体内保持完全复制能力；（2）一种有效的系统，用于在体内罕见细胞中检测和量化病毒启动子活性。本章分为两部分，介绍了我们在体内产生、表征和应用单纯疱疹病毒（HSV）病毒启动子/报告基因突变体的策略和方案。
