Multifluorescence live analysis of herpes simplex virus type-1 replication.
作者信息
Seyffert Michael, de Oliveira Anna Paula, Fraefel Cornel, Vogel Rebecca
机构信息
Institute of Virology, University of Zurich, Winterthurerstr. 266a, 8057, Zurich, Switzerland,
出版信息
Methods Mol Biol. 2014;1144:235-47. doi: 10.1007/978-1-4939-0428-0_16.
PMID:24671688
Abstract
The possibility to label specific viral and cellular structures with live cell markers such as autofluorescent proteins has greatly contributed to our understanding of diverse steps of the virus life cycle, as it allows monitoring virus replication in a spatial and temporal fashion. Here, we describe the multi-fluorescent analysis of the multi-compartment herpes simplex virus type-1 by live-cell confocal laser scanning microscopy.
摘要
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