Kitazawa T, Kudo K, Ishigami M, Furuhashi H, Temma K, Kondo H
Department of Veterinary Pharmacology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.
Naunyn Schmiedebergs Arch Pharmacol. 1988 Jul;338(1):68-73. doi: 10.1007/BF00168814.
The participation of substance P in the noncholinergic contraction induced by transmural stimulation (TMS) of the carp intestinal bulb was examined. In the presence of atropine, substance P caused the contraction of carp intestinal bulb smooth muscle in a concentration dependent manner (1 nmol/l - 1 mumol/l). The EC50 value was 28 +/- 7 nmol/l (n = 6). Substance P-induced desensitization (1 mumol/l for 15 min), decreased the response to substance P and the atropine-resistant contraction induced by TMS (20 Hz) selectively. In contrast, in the absence of atropine, the contraction induced by TMS (20 Hz) was slightly attenuated with the substance P-induced desensitization. The acid extract obtained from the carp intestinal bulb contained a smooth muscle excitatory material whose pharmacological properties were consistent with those of substance P. The present results indicate that a substance P-like peptide is present in the carp intestinal bulb which is involved in the non-cholinergic contraction induced by TMS.
研究了P物质在鲤鱼肠球经壁刺激（TMS）诱导的非胆碱能收缩中的作用。在阿托品存在的情况下，P物质以浓度依赖性方式（1 nmol/l - 1 μmol/l）引起鲤鱼肠球平滑肌收缩。EC50值为28±7 nmol/l（n = 6）。P物质诱导的脱敏（1 μmol/l，持续15分钟）选择性地降低了对P物质的反应以及TMS（20 Hz）诱导的阿托品抵抗性收缩。相反，在没有阿托品的情况下，TMS（20 Hz）诱导的收缩在P物质诱导的脱敏作用下略有减弱。从鲤鱼肠球中获得的酸性提取物含有一种平滑肌兴奋物质，其药理特性与P物质一致。目前的结果表明，鲤鱼肠球中存在一种P物质样肽，它参与了TMS诱导的非胆碱能收缩。
