经壁刺激诱导的鲤鱼（鲤属）肠球部对阿托品抵抗性收缩的药理学特性

Pharmacological properties of the atropine-resistant contraction of the carp (Cyprinus carpio) intestinal bulb induced by transmural stimulation.

Kitazawa T, Furuhashi H, Umezawa K, Ohkoshi N, Temma K, Kondo H

Department of Veterinary Pharmacology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1987;88(2):225-32. doi: 10.1016/0742-8413(87)90114-9.

Abstract

The pharmacological properties of the atropine-resistant contraction of the carp intestinal bulb induced by transmural stimulation were investigated. 2. In the presence of atropine (1 microM), transmural stimulation caused frequency-dependent (2-50 Hz) contraction which was abolished by tetrodotoxin (780 nM). 3. The atropine-resistant contraction was not decreased by tubocurarine (5 microM), hexamethonium (100 microM), carteolol (5 microM) and phentolamine (5.4 microM). 4. In vitro pretreatment with guanethidine (10 microM for 1 hr) markedly decreased the noradrenaline and adrenaline contents of the carp intestinal bulb. The atropine-resistant contraction was not affected by pretreatment with guanethidine. 5. Diphenhydramine (1 microM), methysergide (3 microM) and naloxone (1 microM) did not decrease the atropine-resistant contraction, indicating that histamine, 5-hydroxytryptamine and opioid peptides were not involved in the atropine-resistant response. 6. These results indicate that a non-cholinergic, non-adrenergic excitatory nerve is present in the carp intestinal bulb. The neurotransmitter mediating the excitatory response could not be identified.

摘要

研究了透壁刺激诱导的鲤鱼肠球阿托品抵抗性收缩的药理学特性。2. 在阿托品（1微摩尔）存在的情况下，透壁刺激引起频率依赖性（2 - 50赫兹）收缩，该收缩被河豚毒素（780纳摩尔）消除。3. 筒箭毒碱（5微摩尔）、六甲铵（100微摩尔）、卡替洛尔（5微摩尔）和酚妥拉明（5.4微摩尔）均未降低阿托品抵抗性收缩。4. 用胍乙啶（10微摩尔，处理1小时）进行体外预处理可显著降低鲤鱼肠球中去甲肾上腺素和肾上腺素的含量。胍乙啶预处理对阿托品抵抗性收缩没有影响。5. 苯海拉明（1微摩尔）、甲基麦角新碱（3微摩尔）和纳洛酮（1微摩尔）均未降低阿托品抵抗性收缩，表明组胺、5 - 羟色胺和阿片肽不参与阿托品抵抗反应。6. 这些结果表明，鲤鱼肠球中存在一种非胆碱能、非肾上腺素能的兴奋性神经。介导兴奋性反应的神经递质尚未确定。