Nilsson S, Holmgren S
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1983;76(2):271-6. doi: 10.1016/0742-8413(83)90077-4.
The splanchnic innervation of the stomach of Squalus acanthias has been studied using an isolated perfused stomach preparation and isolated strip preparations from the smooth muscle of the stomach wall. The stomach contracted in response to splanchnic nerve stimulation, and this response was mimicked by all three putative transmitter substances tested: adrenaline, acetylcholine and 5-hydroxytryptamine. The response to nerve stimulation was not affected by atropine (10(-6) M) or methysergide (10(-6) M), but was abolished by phentolamine (10(-6) M). This concentration of phentolamine had little or no effect on the responses of the stomach to exogenous acetylcholine or 5-hydroxytryptamine, but significantly blocked the response to exogenous adrenaline. The results are interpreted in favour of an adrenergic excitatory splanchnic innervation of the dogfish stomach. A "rebound" contraction of the stomach normally occurred upon cessation of the splanchnic nerve stimulation. This response disappeared together with the primary contraction after administration of phentolamine, suggesting the involvement of an adrenergic mechanism in the occurrence of the "rebound" contraction in this preparation. The present experiments did not provide any conclusive evidence for a splanchnic nervous control of the gastric vasculature.
利用分离灌注胃标本和胃壁平滑肌分离条带标本，对棘鲨胃的内脏神经支配进行了研究。胃对内脏神经刺激产生收缩反应，且所测试的三种假定递质物质（肾上腺素、乙酰胆碱和5 - 羟色胺）均可模拟这种反应。对神经刺激的反应不受阿托品（10⁻⁶ M）或麦角新碱（10⁻⁶ M）的影响，但被酚妥拉明（10⁻⁶ M）消除。该浓度的酚妥拉明对胃对外源性乙酰胆碱或5 - 羟色胺的反应几乎没有影响，但显著阻断了胃对外源性肾上腺素的反应。结果表明，犬鲨胃存在肾上腺素能兴奋性内脏神经支配。在内脏神经刺激停止后，胃通常会出现“反弹”收缩。给予酚妥拉明后，这种反应与初级收缩一起消失，表明该制剂中“反弹”收缩的发生涉及肾上腺素能机制。本实验未提供任何确凿证据证明内脏神经对胃血管系统有控制作用。
