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黄芩黄酮醇合酶基因的克隆与鉴定

Cloning and characterization of a flavonol synthase gene from Scutellaria baicalensis.

作者信息

Kim Yeon Bok, Kim KwangSoo, Kim Yeji, Tuan Pham Anh, Kim Haeng Hoon, Cho Jin Woong, Park Sang Un

机构信息

Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of Korea.

Department of Biochemistry and Molecular Biology, Baylor College of Medicine One Baylor Plaza, Houston, TX 77030, USA.

出版信息

ScientificWorldJournal. 2014 Jan 28;2014:980740. doi: 10.1155/2014/980740. eCollection 2014.

DOI:10.1155/2014/980740
PMID:24672406
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3927949/
Abstract

Flavonols are the most abundant of all the flavonoids and play pivotal roles in a variety of plants. We isolated a cDNA clone encoding flavonol synthase from Scutellaria baicalensis (SbFLS). The SbFLS cDNA is 1011 bp long, encodes 336 amino acid residues, and belongs to a family of 2-oxoglutarate-dependent dioxygenases. The overall structure of SbFLS is very similar to that of Arabidopsis thaliana anthocyanidin synthase (AtANS), with a β jelly-roll fold surrounded by tens of short and long α-helices. SbFLS was constitutively expressed in the roots, stems, leaves, and flowers, with particularly high expression in the roots and flowers. SbFLS transcript levels in the roots were 376-, 70-, and 2.5-fold higher than in the leaves, stems, and flowers. The myricetin content was significantly higher than that of kaempferol and quercetin. Therefore, we suggest that SbFLS mediates flavonol formation in the different organs of S. baicalensis. Our study may contribute to the knowledge of the role of FLS in S. baicalensis.

摘要

黄酮醇是所有黄酮类化合物中含量最为丰富的，在多种植物中发挥着关键作用。我们从黄芩中分离出一个编码黄酮醇合酶的cDNA克隆（SbFLS）。SbFLS cDNA长1011 bp，编码336个氨基酸残基，属于2-氧代戊二酸依赖双加氧酶家族。SbFLS的整体结构与拟南芥花青素合酶（AtANS）非常相似，具有由数十个长短不一的α螺旋环绕的β果冻卷折叠结构。SbFLS在根、茎、叶和花中组成型表达，在根和花中的表达尤其高。根中SbFLS转录水平分别比叶、茎和花高376倍、70倍和2.5倍。杨梅素含量显著高于山奈酚和槲皮素。因此，我们认为SbFLS介导黄芩不同器官中黄酮醇的形成。我们的研究可能有助于了解FLS在黄芩中的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2881/3927949/1e0f8ed10b78/TSWJ2014-980740.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2881/3927949/3e63c983e245/TSWJ2014-980740.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2881/3927949/3f11a5a6766c/TSWJ2014-980740.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2881/3927949/f6bfb68e4c18/TSWJ2014-980740.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2881/3927949/f7b34b13db05/TSWJ2014-980740.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2881/3927949/1e0f8ed10b78/TSWJ2014-980740.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2881/3927949/3e63c983e245/TSWJ2014-980740.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2881/3927949/3f11a5a6766c/TSWJ2014-980740.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2881/3927949/f6bfb68e4c18/TSWJ2014-980740.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2881/3927949/f7b34b13db05/TSWJ2014-980740.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2881/3927949/1e0f8ed10b78/TSWJ2014-980740.005.jpg

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