Yuan Yingdan, Zuo Jiajia, Zhang Hanyue, Zu Mengting, Liu Sian
College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China.
Front Nutr. 2022 Sep 26;9:928074. doi: 10.3389/fnut.2022.928074. eCollection 2022.
(L.) Sw. is a valuable herbal crop, and flavonoids are primarily distributed as active ingredients in the stem, but the composition and synthesis mechanisms of flavonoids in different growth years are not clear. The accumulation of flavonoids in from four different years was investigated, using a combined metabolomics and transcriptomics approach in this study. The phenylpropanoid and flavonoid biosynthetic pathways were significantly enriched in the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs). The widely targeted metabolomics technique revealed a total of 173 kinds of flavonoid metabolites. The metabolomics data confirmed the trend of total flavonoids (TF) content in stems of , with chalcone, naringenin, eriodictyol, dihydroquercetin, and other flavonoids considerably up-accumulating in the third year. Twenty DEGs were detected that regulate flavonoid synthesis and the expression of these genes in different growth years was verified using real-time quantitative PCR (qRT-PCR). Furthermore, a comprehensive regulatory network was built for flavonoid biosynthesis and it was discovered that there is one gene, one gene and two MYB transcription factors (TFs) with a high connection with flavonoid biosynthesis by weighted gene co-expression network analysis (WGCNA). In this study, the correlation between genes involved in flavonoid biosynthesis and metabolites was revealed, and a new regulatory mechanism related to flavonoid biosynthesis in was proposed. These results provide an important reference for the farmers involved in the cultivation of .
（L.）Sw.是一种有价值的草本作物，黄酮类化合物主要作为活性成分分布在茎中，但不同生长年份黄酮类化合物的组成和合成机制尚不清楚。本研究采用代谢组学和转录组学相结合的方法，对来自四个不同年份的（植物名称未完整给出）中黄酮类化合物的积累情况进行了研究。在对差异表达基因（DEGs）和差异积累代谢物（DAMs）的京都基因与基因组百科全书（KEGG）富集分析中，苯丙烷类和黄酮类生物合成途径显著富集。广泛靶向代谢组学技术共鉴定出173种黄酮类代谢物。代谢组学数据证实了（植物名称未完整给出）茎中总黄酮（TF）含量的变化趋势，查尔酮、柚皮素、圣草酚、二氢槲皮素等黄酮类化合物在第三年大量积累。检测到20个调控黄酮类化合物合成的DEGs，并通过实时定量PCR（qRT-PCR）验证了这些基因在不同生长年份的表达。此外，构建了黄酮类生物合成的综合调控网络，通过加权基因共表达网络分析（WGCNA）发现有一个（基因名称未完整给出）基因、一个（基因名称未完整给出）基因和两个与黄酮类生物合成高度相关的MYB转录因子（TFs）。本研究揭示了黄酮类生物合成相关基因与代谢物之间的相关性，并提出了一种与（植物名称未完整给出）黄酮类生物合成相关的新调控机制。这些结果为从事（植物名称未完整给出）种植的农民提供了重要参考。
