Smith Brendon W, King Jennifer L, Miller Rita J, Blue James P, Sarwate Sandhya, O'Brien William D, Erdman John W
Bioacoustics Research Laboratory, Department of Electrical and Computer Engineering, University of Illinois, Urbana-Champaign, IL, USA ; Division of Nutritional Sciences, University of Illinois, Urbana-Champaign, IL, USA.
Bioacoustics Research Laboratory, Department of Electrical and Computer Engineering, University of Illinois, Urbana-Champaign, IL, USA.
J Food Res. 2013 Feb 1;2(1):168-178. doi: 10.5539/jfr.v2n1p168.
The cholesterol-fed rabbit is useful for atherosclerosis research. We describe development of a low-magnesium (Mg) cholesterol-containing diet to accelerate atherosclerosis in this model. Male New Zealand White rabbits were fed either chow or one of four atherogenic diets: 1% cholesterol 10% fat 0.11% Mg, 1% cholesterol 10% fat 0.40% Mg, 2% cholesterol 20% fat 0.11% Mg, or 2% cholesterol 20% fat 0.40% Mg. While feed intake decreased in cholesterol-fed rabbits, they were able to maintain their body weights. Rabbits consuming cholesterol experienced profound hypercholesterolemia and tissue lipid accumulation, with plasma cholesterol levels above 1500 mg/dl for all groups at the completion of the study. Liver and spleen lipid content and liver cholesterol content also increased. Aortic arch atheroma thickness was greatest in 1% cholesterol 10% fat 0.11% Mg animals. Tissue Mg levels decreased in cholesterol-fed animals compared to chow-fed controls, despite equal or greater serum Mg levels. Our results indicate that the 1% cholesterol 10% fat 0.11% Mg diet was optimal at promoting hypercholesterolemia and atherosclerosis while minimizing health complications for the animals. The low Mg cholesterol diet will be useful to other biomedical researchers interested in utilizing the rabbit for cardiovascular disease research.
用胆固醇喂养的兔子对动脉粥样硬化研究很有用。我们描述了一种低镁（Mg）含胆固醇饮食的开发，以加速该模型中的动脉粥样硬化。给雄性新西兰白兔喂食普通饲料或四种致动脉粥样硬化饮食之一：1%胆固醇、10%脂肪、0.11%镁，1%胆固醇、10%脂肪、0.40%镁，2%胆固醇、20%脂肪、0.11%镁，或2%胆固醇、20%脂肪、0.40%镁。虽然用胆固醇喂养的兔子采食量减少，但它们能够维持体重。摄入胆固醇的兔子出现了严重的高胆固醇血症和组织脂质积累，在研究结束时所有组的血浆胆固醇水平均高于1500mg/dl。肝脏和脾脏脂质含量以及肝脏胆固醇含量也增加。在1%胆固醇、10%脂肪、0.11%镁组的动物中，主动脉弓动脉粥样瘤厚度最大。与喂食普通饲料的对照组相比，用胆固醇喂养的动物组织镁水平降低，尽管血清镁水平相同或更高。我们的结果表明，1%胆固醇、10%脂肪、0.11%镁的饮食在促进高胆固醇血症和动脉粥样硬化同时将动物健康并发症降至最低方面是最佳的。低镁胆固醇饮食将对其他有兴趣利用兔子进行心血管疾病研究的生物医学研究人员有用。
