Wang Yi-Fei, Li Xin, Xu Rong, Jiang Wen-Cheng, Li Fu-Lun, Ze Kan, Li Bin
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2014 Feb;34(2):218-23.
To study the effect of Shengji Huayu Recipe (SHR)on the expression of MMP-3 and TIMP-1 in the skin ulcer tissue of diabetic rats.
The skin ulcer model was established in diabetic mice. Different compatibility proportions of SHR [the ratio of Shengji Recipe (SJR) to Huayu Recipe (HYR) = 2:1, 1:1, and 1:2, respectively] were used to intervene. The expression of MMP-3 protein in the skin ulcer of diabetic rats was detected by Western blot method,and TIMP-1 protein was detected by immunohistochemical assay.
At each time point, there was no statistical difference in the blood glucose level among groups (P > 0.05). But all of them increased significantly,when compared with those of the normal wound group (P < 0.01). As for the difference between after would area treatment and before would area treatment, better effect was obtained in the SHR No. 3 group and the normal ulcer group than in the diabetic ulcer model group (P < 0.05). Results of Western blot showed that the MMP-3 protein expression was higher in the SHR No. 2 group than in the SHR No.3 group (P < 0.05). Immunohistochemical results showed that TIMP-1 protein expression was lower in the SHR No. 2 group than in the SHR No. 3 group and the diabetic ulcer model group (P < 0.05). TIMP-1 protein expression was higherin the SHR No. 3 group than in the SHR No. 2 group (P < 0.01).
Using SHR No.3 was conducive to the promotion of wound healing in early wound repair stage, and using SHR No. 2 might be conducive to inhibiting the formation of pathological scar.
研究生肌化瘀方（SHR）对糖尿病大鼠皮肤溃疡组织中基质金属蛋白酶-3（MMP-3）和基质金属蛋白酶组织抑制因子-1（TIMP-1）表达的影响。
建立糖尿病小鼠皮肤溃疡模型，采用不同配伍比例的生肌化瘀方[生肌方（SJR）与化瘀方（HYR）的比例分别为2∶1、1∶1、1∶2]进行干预。采用蛋白质免疫印迹法检测糖尿病大鼠皮肤溃疡组织中MMP-3蛋白的表达，免疫组织化学法检测TIMP-1蛋白的表达。
各时间点各组血糖水平比较，差异无统计学意义（P＞0.05），但均较正常创面组显著升高（P＜0.01）。创面面积治疗后与治疗前比较，生肌化瘀方3号组和正常溃疡组效果优于糖尿病溃疡模型组（P＜0.05）。蛋白质免疫印迹法结果显示，生肌化瘀方2号组MMP-3蛋白表达高于生肌化瘀方3号组（P＜0.05）。免疫组织化学结果显示，生肌化瘀方2号组TIMP-1蛋白表达低于生肌化瘀方3号组和糖尿病溃疡模型组（P＜0.05），生肌化瘀方3号组TIMP-1蛋白表达高于生肌化瘀方2号组（P＜0.01）。
生肌化瘀方3号有利于创面修复早期促进创面愈合，生肌化瘀方2号可能有利于抑制病理性瘢痕形成。
