Zhou Xin-Yi, Liu Juan, Wu Qiao-Feng, Tian Xiao-Ning, Fan Ya-Peng, Liu Qi, Du Ping, Tang Yong, Yin Hai-Yan, Yu Shu-Guang
Zhongguo Zhen Jiu. 2014 Jan;34(1):61-5.
To observe different effects of moxibustion on extracellular potassium ion in acupoint under physiological and pathological status and provide experimental evidence for exploring action mechanism of moxibustion on acupoint local.
Forty female SD rats were randomly divided into a blank group, a blank-moxibustion group, a model group and a model-moxibustion group, 10 cases in each one. The complete Freund's adjuvant(CFA) was adopted to establish model of adjuvant arthritis (AA) in the model group and model-moxibustion group. No treatment was given in the blank group and model group while moxibustion was applied at "Zusan-li" (ST 36) for 30 min in the blank-moxibustion group and model-moxibustion group. The tissue fluid in "Zusanli" (ST 36) was collected with microdialysis and real-time analyzed by electrolytic analyzer. The change of concentration of potassium ion in "Zusanli" (ST 36) was observed.
(1) Under physiological status, the concentration of extracellular potassium ion in the blank group was not changed within 150 min (P > 0.05); before the moxibustion, the concentration of extracellular potassium ion in the blank-moxibustion group was (1.21 +/- 0.31) mmol/L, and after treatment it was gradually increased and reached its peak at (2.38 +/- 0.42) mmol/L after 60 min (P < 0.05), then it was reduced. 150 min after the treatment, concentration of potassium ion was slightly higher than that before moxibustion as well as that in the blank group. The concentration in the blank-moxibustion group at 60 min was statistically significant compared with that in the blank group (P < 0.05). (2) Under pathological status, the concentration of extracellular potassium ion in the model group was not changed within 150 min, differences of which at each time point was not statistically significant (all P > 0.05). Before the moxibustion, the concentration of extracellular potassium ion was (1.09 +/- 0.12) mmol/L in the model-moxibustion group, and it was immediately increased to (1.96 +/- 0.18) mmol/L after moxibustion. 60 min and 90 min after the moxibustion, it still maintained a higher level, which was (1.87 +/- 0.29) mmol/L and (1.59 +/- 0.16) mmol/L respectively (both P < 0.05). The differences of each time point after moxibustion in the model-moxibustion group were statistically significant compared with those in the model group (all P < 0.05).
The moxibustion could increase the concentration of potassium ion in rat's acupoint local under physiological status but time of effect is short; with moxibustion at "Zusanli" (ST 36) under pathological status, the concentration of local potassium ion is obviously increased and maintains for a long time.
观察艾灸在生理和病理状态下对穴位细胞外钾离子的不同影响，为探讨艾灸对穴位局部作用机制提供实验依据。
40只雌性SD大鼠随机分为空白组、空白艾灸组、模型组和模型艾灸组，每组10只。模型组和模型艾灸组采用完全弗氏佐剂（CFA）建立佐剂性关节炎（AA）模型。空白组和模型组不做处理，空白艾灸组和模型艾灸组于“足三里”（ST36）施灸30分钟。用微透析法采集“足三里”（ST36）组织液，用电解质分析仪实时分析，观察“足三里”（ST36）钾离子浓度变化。
（1）生理状态下，空白组细胞外钾离子浓度在150分钟内无变化（P>0.05）；艾灸前，空白艾灸组细胞外钾离子浓度为（1.21±0.31）mmol/L，处理后逐渐升高，60分钟时达到峰值（2.38±0.42）mmol/L（P<0.05），随后降低。处理150分钟后，钾离子浓度略高于艾灸前及空白组。空白艾灸组60分钟时浓度与空白组比较差异有统计学意义（P<0.05）。（2）病理状态下，模型组细胞外钾离子浓度在150分钟内无变化，各时间点差异无统计学意义（均P>0.05）。艾灸前，模型艾灸组细胞外钾离子浓度为（1.09±0.12）mmol/L，艾灸后立即升至（1.96±0.18）mmol/L。艾灸后60分钟和90分钟仍维持较高水平，分别为（1.87±0.29）mmol/L和（1.59±0.16）mmol/L（均P<0.05）。模型艾灸组艾灸后各时间点与模型组比较差异有统计学意义（均P<0.05）。
生理状态下艾灸可使大鼠穴位局部钾离子浓度升高，但作用时间较短；病理状态下艾灸“足三里”（ST36），局部钾离子浓度明显升高且维持时间较长。
