Vicente Sara, Peleteiro Mercedes, Gonzalez-Aramundiz Jose V, Díaz-Freitas Belén, Martínez-Pulgarín Susana, Neissa Jose I, Escribano Jose M, Sanchez Alejandro, González-Fernández Africa, Alonso Maria J
Center for Research in Molecular Medicine & Chronic Diseases (CIMUS), University of Santiago de Compostela, 15706 Campus Vida, Santiago de Compostela, Spain and Pharmacy & Pharmaceutical Technology Department, School of Pharmacy, University of Santiago de Compostela, 15705 Campus Vida, Santiago de Compostela, Spain and Current affiliation: Exploratory Unit, Sanofi-Aventis R&D, 31036 Toulouse, France.
Nanomedicine (Lond). 2014 Oct;9(15):2273-89. doi: 10.2217/nnm.14.10. Epub 2014 Mar 27.
To develop a new core-shell type (nanocapsules) adjuvant system composed of squalene and polyglucosamine (PG) and to evaluate its immunostimulant capacity.
The defined PG nanocapsules exhibited the capacity to efficiently associate the selected antigens (recombinant hepatitis B surface antigen and hemagglutinin of influenza virus) onto their polymeric surface (70-75%), and the immunostimulant imiquimod within the oily core. The resulting nanovaccines, with a particle size of 200-250 nm and a positive zeta-potential (∼+60 mV), were able to significantly potentiate and modulate the immune response to the selected antigens upon intramuscular administration to mice. Their efficacy as novel adjuvants was attributed to their enhanced cell internalization and effective intracellular imiquimod/antigen delivery, together with their prolonged residence time at the injection site.
The nanocapsules described herein have the capacity to enhance, prolong and modulate the immune response of subunit antigens and, therefore, they could be proposed as a platform for the codelivery of different antigens and immunostimulators.
开发一种由角鲨烯和聚葡糖胺（PG）组成的新型核壳型（纳米胶囊）佐剂系统，并评估其免疫刺激能力。
确定的PG纳米胶囊能够有效地将选定的抗原（重组乙肝表面抗原和流感病毒血凝素）结合到其聚合物表面（70 - 75%），并将免疫刺激剂咪喹莫特包封在油相核心内。所得纳米疫苗的粒径为200 - 250 nm，zeta电位为正（约 +60 mV），经肌肉注射给小鼠后，能够显著增强和调节对选定抗原的免疫反应。它们作为新型佐剂的功效归因于其增强的细胞内化作用、有效的细胞内咪喹莫特/抗原递送，以及在注射部位延长的停留时间。
本文所述的纳米胶囊能够增强、延长和调节亚单位抗原的免疫反应，因此，它们可被提议作为不同抗原和免疫刺激剂共递送的平台。
