Department of Chemistry, NSBI, Dankook University , 126 Jukjeon-dong, Suji-gu, Yongin-si, Gyeonggi-do 448-701, Korea.
Anal Chem. 2014 May 6;86(9):4140-4. doi: 10.1021/ac4036668. Epub 2014 Apr 8.
Multicore magnetic nanoparticles (MMNPs) doped with Cs and FITC (Cs/FITC-doped MMNPs) were synthesized for the extraction and determination of biomarkers using inductively coupled plasma-mass spectrometry (ICP-MS). For demonstration, the MMNPs were used for magnetic separation to extract CA19-9 in serum nonspecifically, and the doped Cs was used as an internal standard for the ratiometric measurement of the tagged particle. This ratiometric method compensated for the particle loss in a magnetic separation and suppressed the signal fluctuation which increased the calibration linearity significantly. The obtained detection limit was 0.02 units/mL of CA19-9, which is more than 300 times lower than that reported by the ICP-MS with element tagging and about 500-fold improved compared to ELISA.
多芯磁性纳米粒子（MMNPs）掺杂铯和 FITC（Cs/FITC 掺杂 MMNPs）被合成，用于使用电感耦合等离子体质谱法（ICP-MS）提取和测定生物标志物。为了演示，MMNPs 被用于非特异性地从血清中磁性分离提取 CA19-9，掺杂的 Cs 被用作标记粒子的相对测量的内标。这种相对测量方法补偿了磁性分离中的粒子损失，并抑制了信号波动，从而大大提高了校准线性度。获得的检测限为 CA19-9 的 0.02 个单位/mL，比 ICP-MS 元素标记报道的检测限低 300 多倍，比 ELISA 提高了约 500 倍。
