Department of Chemistry, Dankook University, Yongin-si, Gyeonggi-do 448-701, Republic of Korea.
Department of Chemistry, Dankook University, Yongin-si, Gyeonggi-do 448-701, Republic of Korea.
Talanta. 2018 Feb 1;178:916-921. doi: 10.1016/j.talanta.2017.10.009. Epub 2017 Oct 12.
In this work, a magnetophoretic separation ICP-MS immunoassay using newly synthesized multicore magnetic nanoparticles (MMNPs) was developed for the determination of salmonella typhimurium (typhi). The uniqueness of this method was the use of MMNPs doped with Cs for both separation and detection, which enable us to achieve fast analysis, high sensitivity, and good reliability. For demonstration, heat-killed typhi in a phosphate buffer solution was determined by ICP-MS after the MMNP-typhi reaction product was separated from unreacted MMNPs in a micropipette tip filled with 25% polyethylene glycol through magnetophoretic separation. The calibration curve obtained by plotting Cs intensity vs. the number of synthetic standard, showed a coefficient of determination (R) of 0.94 with a limit of detection (LOD) of 102 cells/mL without cell culturing. Excellent recoveries, between 98-100%, were obtained from four replicates and compared with a sandwich-type ICP-MS immunoassay for further confirmation.
在这项工作中，开发了一种基于新合成多核磁性纳米粒子（MMNPs）的磁泳分离 ICP-MS 免疫分析方法，用于检测鼠伤寒沙门氏菌（伤寒）。该方法的独特之处在于使用掺杂 Cs 的 MMNPs 进行分离和检测，这使我们能够实现快速分析、高灵敏度和良好的可靠性。为了进行演示，在通过磁泳分离从填充有 25%聚乙二醇的微吸管尖端中分离未反应的 MMNPs 后，将磷酸盐缓冲溶液中的热灭活伤寒用 ICP-MS 测定 MMNP-伤寒反应产物。通过绘制 Cs 强度与合成标准品数量的关系曲线，得到了一个决定系数（R）为 0.94，检测限（LOD）为 102 个细胞/mL，无需细胞培养。从四个重复中获得了 98-100%的优异回收率，并与三明治型 ICP-MS 免疫分析进行了比较，以进一步确认。
