Future Environmental Research Center, Korea Institute of Toxicology, Jinju, 660-844, South Korea; Department of Chemistry, Dankook University, Yongin-si, Gyeonggi-do, 448-701, South Korea.
Department of Chemistry, Dankook University, Yongin-si, Gyeonggi-do, 448-701, South Korea.
Anal Chim Acta. 2016 Sep 28;938:1-6. doi: 10.1016/j.aca.2016.07.035. Epub 2016 Jul 28.
Metal-doped inorganic nanoparticles were synthesized for the multiplex detection of biomarkers by a sandwich-type inductively coupled plasma mass spectrometry (ICP-MS) immunoassay. The synthesized Cs-doped multicore magnetic nanoparticles (MMNPs) were used not only for magnetic extraction of targets but also for ratiometric measurement in ICP-MS. In addition, three different metal/dye-doped silica nanoparticles (SNPs) were synthesized as probes for multiplex detection: Y/RhBITC (rhodamine B isothiocyanate)-doped SNPs for CRP (cardiovascular disease), Cd/RhBITC-doped SNPs for AFP (tumor), and Au/5(6)-XRITC (X-rhodamine-5-(and-6)-isothiocyanate)-doped SNPs for NSE (heart disease). For quantification, the doped metals of SNPs were measured by ICP-MS and then the signal ratio to Cs of MMNPs was plotted with respect to the concentration of targets by a ratiometry. Limits of detection (LOD) of 0.35 ng/mL to 77 ng mL(-1) and recoveries of 83%-125% were obtained for serum samples spiked with the biomarkers. Since no sample treatment was necessary prior to the extraction, the proposed method provided short analysis time and convenience for the multiplex determination of biomarkers, which will be valuable for clinical application.
采用三明治型电感耦合等离子体质谱（ICP-MS）免疫分析法，通过合成金属掺杂无机纳米粒子实现了生物标志物的多重检测。所合成的铯掺杂多核磁性纳米粒子（MMNPs）不仅用于目标物的磁性萃取，还用于 ICP-MS 中的比率测量。此外，还合成了三种不同的金属/染料掺杂二氧化硅纳米粒子（SNPs）作为多重检测的探针：用于 CRP（心血管疾病）检测的 Y/RhBITC（罗丹明 B 异硫氰酸酯）-掺杂 SNPs、用于 AFP（肿瘤）检测的 Cd/RhBITC-掺杂 SNPs，以及用于 NSE（心脏病）检测的 Au/5(6)-XRITC（X-罗丹明 5-(和-6)-异硫氰酸酯）-掺杂 SNPs。对于定量，通过 ICP-MS 测量 SNPs 中的掺杂金属，然后通过比率法绘制 MMNPs 相对于目标物浓度的信号与 Cs 的比值。对于用生物标志物加标的血清样品，该方法的检测限（LOD）为 0.35 ng/mL 至 77 ng/mL，回收率为 83%-125%。由于在提取之前不需要进行样品处理，因此该方法为生物标志物的多重测定提供了短的分析时间和便利，这对于临床应用将具有重要价值。
