Li Lei, Lv Xiaoqing, Ostrovidov Serge, Shi Xuetao, Zhang Ning, Liu Jing
Key Laboratory of Cryogenics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences & Beijing Key Laboratory of Cryo-Biomedical Engineering , Beijing100190, China.
Mol Pharm. 2014 Jul 7;11(7):2009-15. doi: 10.1021/mp5000532. Epub 2014 Apr 7.
Microfluidic devices have emerged as revolutionary, novel platforms for in vitro drug evaluation. In this work, we developed a facile method for evaluating antihypertensive drugs using a microfluidic chip. This microfluidic chip was generated using the elastic material poly(dimethylsiloxane) (PDMS) and a microchannel structure that simulated a blood vessel as fabricated on the chip. We then cultured human umbilical vein endothelial cells (HUVECs) inside the channel. Different pressures and shear stresses could be applied on the cells. The generated vessel mimics can be used for evaluating the safety and effects of antihypertensive drugs. Here, we used hydralazine hydrochloride as a model drug. The results indicated that hydralazine hydrochloride effectively decreased the pressure-induced dysfunction of endothelial cells. This work demonstrates that our microfluidic system provides a convenient and cost-effective platform for studying cellular responses to drugs under mechanical pressure.
微流控装置已成为用于体外药物评估的革命性新型平台。在这项工作中，我们开发了一种使用微流控芯片评估抗高血压药物的简便方法。该微流控芯片由弹性材料聚二甲基硅氧烷（PDMS）制成，并在芯片上构建了模拟血管的微通道结构。然后我们在通道内培养人脐静脉内皮细胞（HUVECs）。可以对细胞施加不同的压力和剪切应力。所生成的血管模拟物可用于评估抗高血压药物的安全性和效果。在此，我们使用盐酸肼屈嗪作为模型药物。结果表明，盐酸肼屈嗪有效地降低了压力诱导的内皮细胞功能障碍。这项工作表明，我们的微流控系统为研究机械压力下细胞对药物的反应提供了一个方便且经济高效的平台。
