酵母中蛋白质的共免疫沉淀
Coimmunoprecipitation of proteins from yeast.
作者信息
Gerace Erica, Moazed Danesh
机构信息
Department of Cell Biology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA, USA.
Department of Cell Biology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA, USA.
出版信息
Methods Enzymol. 2014;541:13-26. doi: 10.1016/B978-0-12-420119-4.00002-1.
Abstract
This protocol outlines a procedure for testing whether two proteins interact. A target protein will be immunoprecipitated using an antibody that recognizes it (or a tagged version of the protein). The immunoprecipitated material will be separated by SDS-PAGE and analyzed by Western blotting to assess the presence of a candidate interacting protein(s).
摘要
本方案概述了一种检测两种蛋白质是否相互作用的程序。将使用识别目标蛋白质（或该蛋白质的标签化版本）的抗体对目标蛋白质进行免疫沉淀。免疫沉淀的物质将通过SDS-PAGE分离，并通过蛋白质印迹法进行分析，以评估候选相互作用蛋白质的存在情况。
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