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酵母中蛋白质的共免疫沉淀

Coimmunoprecipitation of proteins from yeast.

作者信息

Gerace Erica, Moazed Danesh

机构信息

Department of Cell Biology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA, USA.

Department of Cell Biology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA, USA.

出版信息

Methods Enzymol. 2014;541:13-26. doi: 10.1016/B978-0-12-420119-4.00002-1.

DOI:10.1016/B978-0-12-420119-4.00002-1
PMID:24674059
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4370295/
Abstract

This protocol outlines a procedure for testing whether two proteins interact. A target protein will be immunoprecipitated using an antibody that recognizes it (or a tagged version of the protein). The immunoprecipitated material will be separated by SDS-PAGE and analyzed by Western blotting to assess the presence of a candidate interacting protein(s).

摘要

本方案概述了一种检测两种蛋白质是否相互作用的程序。将使用识别目标蛋白质（或该蛋白质的标签化版本）的抗体对目标蛋白质进行免疫沉淀。免疫沉淀的物质将通过SDS-PAGE分离，并通过蛋白质印迹法进行分析，以评估候选相互作用蛋白质的存在情况。

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Mol Cell. 2010 Aug 13;39(3):360-72. doi: 10.1016/j.molcel.2010.07.017.
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Ethidium bromide provides a simple tool for identifying genuine DNA-independent protein associations.溴化乙锭为鉴定真正的非DNA依赖性蛋白质结合提供了一种简单的工具。
Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):6958-62. doi: 10.1073/pnas.89.15.6958.
中的新功能化：一种新型的Nrg1-Rtg3嵌合转录调节因子对于维持线粒体DNA完整性至关重要。
R Soc Open Sci. 2023 Nov 1;10(11):231209. doi: 10.1098/rsos.231209. eCollection 2023 Nov.
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Mitotically heritable, RNA polymerase II-independent H3K4 dimethylation stimulates transcriptional memory.具有有丝分裂遗传性、不依赖 RNA 聚合酶 II 的 H3K4 二甲基化可刺激转录记忆。
Elife. 2022 May 17;11:e77646. doi: 10.7554/eLife.77646.
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Gibberellin and auxin signaling genes RGA1 and ARF8 repress accessory fruit initiation in diploid strawberry.赤霉素和生长素信号基因 RGA1 和 ARF8 抑制二倍体草莓附属果实的起始。
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