Food Safety and Animal Health Division, Alberta Agriculture and Rural Development, O.S. Longman Building, 6909-116 Street, Edmonton, Alberta, Canada T6H 4P2.
Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, 160 Research Lane, Unit 103, Guelph, Ontario, Canada N1G 5B2.
J Food Prot. 2014 Mar;77(3):485-92. doi: 10.4315/0362-028X.JFP-13-363.
Antimicrobial-resistant Salmonella species are threatening to become a serious public health problem. Therefore, surveillance and prudent use of antimicrobials is needed in both the agricultural and human health sectors. The aim of this study was to describe the antimicrobial susceptibility profiles of Salmonella isolates recovered from healthy broiler chickens at slaughter from November 2004 to April 2005. Salmonella isolates recovered from 36 broiler flocks in Alberta, Canada, were serotyped and tested for antimicrobial susceptibility against 15 antimicrobials. Of 272 Salmonella isolates tested, 64.0% were resistant to one or more antimicrobials, 10.0% were resistant to three or more antimicrobials, and 1.8% were resistant to five antimicrobials. All isolates were susceptible to amikacin, amoxicillin-clavulanic acid, ceftiofur, cefoxitin, ceftriaxone, ciprofloxacin, and nalidixic acid. The highest prevalence of resistance was to tetracycline (54.8%), followed by streptomycin (24.2%) and sulfisoxazole (8.4%). The most common multiantimicrobial resistance patterns were to streptomycin-tetracycline (24.3%), streptomycin-sulfisoxazole-tetracycline (6.6%), and ampicillin-streptomycin-sulfisoxazole-tetracycline (3.7%). The strongest associations were observed between resistance to kanamycin and tetracycline (odds ratio = 65.7, P = 0.001) and to ampicillin and sulfisoxazole (odds ratio = 62.9, P = 0.001). Salmonella Hadar and Salmonella Heidelberg were the two most common serovars accounting for 40.4 and 13.6% of the total isolates, respectively. Eighty-one percent and 12.7% of Salmonella Hadar isolates and 62.0 and 8.1% of Salmonella Heidelberg isolates were resistant to 1 or more and three or more antimicrobials, respectively. The flock level prevalence of resistance ranged from 5.6% for trimethoprim-sulfamethoxazole to 83.3% for tetracycline. This study provides baseline information on antimicrobial susceptibility of Salmonella isolates of broiler chickens at slaughter in Alberta that can serve as a benchmark for future research.
耐抗生素的沙门氏菌物种正威胁成为严重的公共卫生问题。因此，在农业和人类健康领域都需要对微生物进行监测和谨慎使用。本研究的目的是描述 2004 年 11 月至 2005 年 4 月从屠宰的健康肉鸡中分离出的沙门氏菌的抗生素药敏谱。从加拿大艾伯塔省的 36 个肉鸡群中分离出沙门氏菌，对其进行血清分型，并检测 15 种抗生素的药敏性。在 272 株沙门氏菌中，64.0%对一种或多种抗生素耐药，10.0%对三种或更多抗生素耐药，1.8%对五种抗生素耐药。所有分离株均对阿米卡星、阿莫西林-克拉维酸、头孢噻呋、头孢西丁、头孢曲松、环丙沙星和萘啶酸敏感。耐药率最高的是四环素（54.8%），其次是链霉素（24.2%）和磺胺二甲嘧啶（8.4%）。最常见的多药耐药模式是链霉素-四环素（24.3%）、链霉素-磺胺二甲嘧啶-四环素（6.6%）和氨苄西林-链霉素-磺胺二甲嘧啶-四环素（3.7%）。对卡那霉素和四环素的耐药性之间存在最强的相关性（比值比=65.7，P=0.001），对氨苄西林和磺胺二甲嘧啶的耐药性之间也存在最强的相关性（比值比=62.9，P=0.001）。沙门氏菌哈达和沙门氏菌海德堡是两种最常见的血清型，分别占总分离株的 40.4%和 13.6%。沙门氏菌哈达的 81%和 12.7%的分离株以及沙门氏菌海德堡的 62.0%和 8.1%的分离株分别对 1 种或多种和 3 种或多种抗生素耐药。在鸡群水平上，对甲氧苄啶-磺胺甲恶唑的耐药率为 5.6%，对四环素的耐药率为 83.3%。本研究提供了艾伯塔省屠宰肉鸡中沙门氏菌的抗生素药敏谱的基线信息，可作为未来研究的基准。
