Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, 120-255 Woodlawn Road West, Guelph, Ontario, Canada N1H 8J1.
J Food Prot. 2011 Aug;74(8):1245-51. doi: 10.4315/0362-028X.JFP-10-483.
This study estimated the prevalence of Salmonella, Campylobacter, and Escherichia coli isolates in fresh retail grain-fed veal obtained in Ontario, Canada. The prevalence and antimicrobial resistance patterns were examined for points of public health significance. Veal samples (n = 528) were collected from February 2003 through May 2004. Twenty-one Salmonella isolates were recovered from 18 (4%) of 438 samples and underwent antimicrobial susceptibility testing. Resistance to one or more antimicrobials was found in 6 (29%) of 21 Salmonella isolates; 5 (24%) of 21 isolates were resistant to five or more antimicrobials. No resistance to antimicrobials of very high human health importance was observed. Ampicillin-chloramphenicolstreptomycin-sulfamethoxazole-tetracycline resistance was found in 5 (3%) of 21 Salmonella isolates. Campylobacter isolates were recovered from 5 (1%) of 438 samples; 6 isolates underwent antimicrobial susceptibility testing. Resistance to one or more antimicrobials was documented in 3 (50%) of 6 Campylobacter isolates. No Campylobacter isolates were resistant to five or more antimicrobials or category I antimicrobials. E. coli isolates were recovered from 387 (88%) of 438 samples; 1,258 isolates underwent antimicrobial susceptibility testing. Resistance to one or more antimicrobials was found in 678 (54%) of 1,258 E. coli isolates; 128 (10%) of 1,258 were resistant to five or more antimicrobials. Five (0.4%) and 7 (0.6%) of 1,258 E. coli isolates were resistant to ceftiofur and ceftriaxone, respectively, while 34 (3%) of 1,258 were resistant to nalidixic acid. Ciprofloxacin resistance was not detected. There were 101 different resistance patterns observed among E. coli isolates; resistance to tetracycline alone (12.7%, 161 of 1,258) was most frequently observed. This study provides baseline prevalence and antimicrobial resistance data and highlights potential public health concerns.
本研究估计了在加拿大安大略省新鲜零售谷物喂养小牛肉中分离出的沙门氏菌、弯曲杆菌和大肠杆菌的流行率。研究检查了与公共卫生相关的重点的流行率和抗生素耐药模式。2003 年 2 月至 2004 年 5 月期间采集了 528 份小牛肉样本。从 438 份样本中的 18 份（4%）中分离出 21 株沙门氏菌，并进行了抗生素药敏试验。21 株沙门氏菌中有 6 株（29%）对一种或多种抗生素耐药；21 株中有 5 株（24%）对 5 种或更多种抗生素耐药。未观察到对非常重要的人类健康抗生素的耐药性。21 株沙门氏菌中有 5 株（3%）对氨苄西林-氯霉素-链霉素-磺胺甲噁唑-四环素耐药。从 438 份样本中的 5 份（1%）中分离出弯曲杆菌；对 6 株进行了抗生素药敏试验。6 株弯曲杆菌中有 3 株（50%）对一种或多种抗生素耐药。未发现对 5 种或更多种抗生素或 I 类抗生素耐药的弯曲杆菌。从 438 份样本中的 387 份（88%）中分离出大肠杆菌；对 1258 株进行了抗生素药敏试验。1258 株大肠杆菌中有 678 株（54%）对一种或多种抗生素耐药；1258 株中有 128 株（10%）对 5 种或更多种抗生素耐药。1258 株大肠杆菌中有 5 株（0.4%）和 7 株（0.6%）分别对头孢噻呋和头孢曲松耐药，34 株（3%）对萘啶酸耐药。未检测到环丙沙星耐药性。在大肠杆菌分离株中观察到 101 种不同的耐药模式；单独对四环素的耐药率最高（12.7%，161 株）。本研究提供了基线流行率和抗生素耐药数据，并强调了潜在的公共卫生问题。
