Siddiky Nure Alam, Sarker Md Samun, Khan Md Shahidur Rahman, Begum Ruhena, Kabir Md Ehsanul, Karim Md Rezaul, Rahman Md Tanvir, Mahmud Asheak, Samad Mohammed A
Antimicrobial Resistance Action Center, Bangladesh Livestock Research Institute, Savar, Dhaka 1341, Bangladesh.
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.
Microorganisms. 2021 Apr 28;9(5):952. doi: 10.3390/microorganisms9050952.
Virulent and multi drug resistant (MDR) is a foremost cause of foodborne diseases and had serious public health concern globally. The present study was undertaken to identify the pathogenicity and antimicrobial resistance (AMR) profiles of serovars recovered from chicken at wet markets in Dhaka, Bangladesh. A total of 870 cecal contents of broiler, sonali, and native chickens were collected from 29 wet markets. The overall prevalence of Typhimurium, Enteritidis, and untyped spp., were found to be 3.67%, 0.57%, and 1.95% respectively. All isolates were screened by polymerase chain reaction (PCR) for eight virulence genes, namely A, A, A, A, H, A, E, and C. . Enteritidis isolates carried all virulence genes whilst . Typhimurium isolates carried six virulence genes except A and C. A diverse phenotypic and genotypic AMR pattern was found. Harmonic descending trends of resistance patterns were observed among the broiler, sonali, and native chickens. Interestingly, virulent and MDR serovars were found in native chicken, although antimicrobials were not used in their production cycle. The research findings anticipate that virulent and MDR are roaming in the wet markets which can easily anchor to the vendor, consumers, and in the food chain.
致病性和多重耐药性是食源性疾病的首要原因，在全球范围内引起了严重的公共卫生关注。本研究旨在确定从孟加拉国达卡湿货市场的鸡肉中分离出的血清型的致病性和抗菌药物耐药性（AMR）谱。从29个湿货市场共收集了870份肉鸡、索纳利鸡和本地鸡的盲肠内容物。鼠伤寒沙门氏菌、肠炎沙门氏菌和未分型菌株的总体患病率分别为3.67%、0.57%和1.95%。所有分离株均通过聚合酶链反应（PCR）筛选了8个毒力基因，即A、A、A、A、H、A、E和C。肠炎沙门氏菌分离株携带所有毒力基因，而鼠伤寒沙门氏菌分离株除A和C外携带6个毒力基因。发现了多种表型和基因型的AMR模式。在肉鸡、索纳利鸡和本地鸡中观察到耐药模式呈谐波下降趋势。有趣的是，在本地鸡中发现了致病性和多重耐药性血清型，尽管在其生产周期中未使用抗菌药物。研究结果预计，致病性和多重耐药性血清型在湿货市场中存在，它们很容易附着于摊贩、消费者以及食物链中。
