Irving Amy A, Young Lindsay B, Pleiman Jennifer K, Konrath Michael J, Marzella Blake, Nonte Michael, Cacciatore Justin, Ford Madeline R, Clipson Linda, Amos-Landgraf James M, Dove William F
McArdle Laboratory for Cancer Research, Department of Oncology, Molecular and Environmental Toxicology Center, University of Wisconsin-Madison, Madison, Wisconsin, USA.
McArdle Laboratory for Cancer Research, Department of Oncology, Laboratory of Genetics, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Comp Med. 2014 Apr;64(2):128-34.
Many studies of the response of colonic tumors to therapeutics use tumor multiplicity as the endpoint to determine the effectiveness of the agent. These studies can be greatly enhanced by accurate measurements of tumor volume. Here we present a quantitative method to easily and accurately determine colonic tumor volume. This approach uses a biocompatible alginate to create a negative mold of a tumor-bearing colon; this mold is then used to make positive casts of dental stone that replicate the shape of each original tumor. The weight of the dental stone cast correlates highly with the weight of the dissected tumors. After refinement of the technique, overall error in tumor volume was 16.9% ± 7.9% and includes error from both the alginate and dental stone procedures. Because this technique is limited to molding of tumors in the colon, we utilized the Apc(Pirc/+) rat, which has a propensity for developing colonic tumors that reflect the location of the majority of human intestinal tumors. We have successfully used the described method to determine tumor volumes ranging from 4 to 196 mm³. Alginate molding combined with dental stone casting is a facile method for determining tumor volume in vivo without costly equipment or knowledge of analytic software. This broadly accessible method creates the opportunity to objectively study colonic tumors over time in living animals in conjunction with other experiments and without transferring animals from the facility where they are maintained.
许多关于结肠肿瘤对治疗反应的研究将肿瘤的多发性作为终点来确定药物的有效性。通过准确测量肿瘤体积，这些研究可以得到极大的加强。在此，我们提出一种定量方法，可轻松准确地测定结肠肿瘤体积。该方法使用生物相容性藻酸盐制作携带肿瘤结肠的阴性模具；然后使用该模具制作牙科石膏的阳性铸型，以复制每个原始肿瘤的形状。牙科石膏铸型的重量与解剖后肿瘤的重量高度相关。对该技术进行改进后，肿瘤体积的总体误差为16.9%±7.9%，且包括藻酸盐和牙科石膏操作的误差。由于该技术仅限于对结肠中的肿瘤进行成型，我们使用了Apc(Pirc/+)大鼠，该大鼠易于发生结肠肿瘤，其肿瘤位置反映了大多数人类肠道肿瘤的位置。我们已成功使用所述方法测定了4至196立方毫米范围内的肿瘤体积。藻酸盐成型结合牙科石膏铸型是一种在体内测定肿瘤体积的简便方法，无需昂贵设备或分析软件知识。这种广泛适用的方法创造了机会，可在活体动物中随着时间推移结合其他实验客观地研究结肠肿瘤，而无需将动物转移到饲养它们的设施之外。
