Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, PR China.
Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, PR China; Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing 210009, PR China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China.
J Chromatogr A. 2014 Apr 25;1339:185-91. doi: 10.1016/j.chroma.2014.02.003. Epub 2014 Feb 8.
Nanoparticles (NPs) and ionic liquids (ILs) have been extensively studied and have aroused considerable interest in separation science; however, the employment of ILs-dispersed NPs as buffer modifiers for CE chiral separation has not been previously studied. In this work, we describe a new CE method using ILs dispersed multi-walled carbon nanotubes (ILs-MWNTs) as a modifier for enantioseparation with a polysaccharide, chondroitin sulfate E (CSE), as the chiral selector. As observed, significantly improved separations, including better enantioselectivity and improved peak shapes, were obtained in the ILs-MWNTs modified separation system for all drug enantiomers compared to the single CSE system. Several parameters affecting the enantioseparation, such as the choice of ILs and carbon nanoparticles, ILs-MWNTs concentration, chiral selector concentration, buffer pH and applied voltage, were systematically investigated. Satisfactory separations were achieved when 2.4μg/mL ILs-MWNTs were introduced into the 20mM Tris/H3PO4 buffer solution containing 2.5% CSE at pH 2.8-3.4 with a 15kV applied voltage. A brief mechanism of the enhanced enantioseparation capability of the ILs-MWNTs modified chiral separation system was also discussed.
纳米粒子（NPs）和离子液体（ILs）已经得到了广泛的研究，并在分离科学中引起了相当大的兴趣；然而，将 ILs 分散的 NPs 用作 CE 手性分离的缓冲修饰剂尚未被研究过。在这项工作中，我们描述了一种新的 CE 方法，使用离子液体分散的多壁碳纳米管（ILs-MWNTs）作为修饰剂，以多糖硫酸软骨素 E（CSE）作为手性选择剂进行对映体分离。如观察到的，与单一 CSE 体系相比，所有药物对映体在 ILs-MWNTs 修饰分离体系中得到了显著改善的分离，包括更好的对映选择性和改善的峰形。系统研究了影响对映体分离的几个参数，如 ILs 和碳纳米粒子的选择、ILs-MWNTs 浓度、手性选择剂浓度、缓冲液 pH 值和施加电压。当在 pH 值为 2.8-3.4 的 20mM Tris/H3PO4 缓冲溶液中引入 2.4μg/mL 的 ILs-MWNTs 并含有 2.5% CSE 时，在施加 15kV 电压下可实现令人满意的分离。还讨论了 ILs-MWNTs 修饰手性分离体系增强对映体分离能力的简要机制。
