Costa Marilza S, Cossolin Jamile F S, Pereira Mônica J B, Sant'Ana Antônio E G, Lima Milena D, Zanuncio José C, Serrão José Eduardo
Laboratory of Cell Ultrastructure, Department of General Biology, Federal University of Viçosa, Viçosa 36570-000, Minas Gerais, Brazil.
Laboratory of Entomology, Research and Study Center of Agriculture and environment Development, University of Mato Grosso State; MT 358, Km 7, Jardim Aeroporto, Tangará da Serra 78300-000, Mato Grosso, Brazil.
Toxins (Basel). 2014 Mar 26;6(4):1169-76. doi: 10.3390/toxins6041169.
Acetogenins are secondary metabolites exclusively produced by Annonaceae, which have antitumor, cytotoxic, and pesticide activities. In this study, we evaluated the larvicidal and cytotoxic effect of squamocin from Annona squamosa on Aedes aegypti (Diptera: Culicidae) midgut. The compound was solubilized in 2% Tween 20 at 10, 20, 50, 80 and 100 ppm. The assay was conducted in a completely randomized design with four replications, each with 20 third-instar larvae. Larval mortality was assessed every hour until total mortality, and the data were subjected to Probit analysis. Cellular damage was evaluated every 30 min in groups comprising five larvae subjected to squamocin at 50 and 100 ppm for 240 min. The total larval mortality occurred after 360 min following application of 50, 80, and 100 ppm squamocin, and 600 min after applying other concentrations with LC50 at 6.4 ppm. Both 50 and 100 ppm of squamocin showed cytotoxic activity in the midgut epithelium of A. aegypti after 240 min with 50 ppm resulting in midgut cells with light cytoplasm containing small vacuoles, whereas at 100 ppm were found cells with cytoplasm highly vacuolated, damaged apical surface and cell protrusion toward the gut lumen. In conclusion, squamocin has the potential to control A. aegypti.
番荔枝内酯是番荔枝科植物特有的次生代谢产物，具有抗肿瘤、细胞毒性和杀虫活性。在本研究中，我们评估了番荔枝叶中番荔枝辛对埃及伊蚊（双翅目：蚊科）中肠的杀幼虫和细胞毒性作用。该化合物以10、20、50、80和100 ppm的浓度溶解于2%吐温20中。试验采用完全随机设计，重复4次，每次20只三龄幼虫。每小时评估幼虫死亡率直至全部死亡，并对数据进行概率分析。在由5只幼虫组成的组中，每30分钟评估一次细胞损伤，这些幼虫分别在50和100 ppm的番荔枝辛作用下处理240分钟。在施用50、80和100 ppm的番荔枝辛后360分钟出现全部幼虫死亡，施用其他浓度后600分钟全部死亡，LC50为6.4 ppm。在处理240分钟后，50和100 ppm的番荔枝辛均在埃及伊蚊中肠上皮细胞中表现出细胞毒性活性，50 ppm导致中肠细胞的细胞质轻度空泡化，而100 ppm时发现细胞的细胞质高度空泡化，顶端表面受损且细胞向肠腔突出。总之，番荔枝辛具有控制埃及伊蚊的潜力。
