Ankara and Nigde, Turkey From the Departments of Plastic Reconstructive and Aesthetic Surgery, Histology and Embryology, and Anatomy, Hacettepe University Faculty of Medicine; the Chemical Engineering and Bioengineering Departments, Hacettepe University; and the Department of Geological Engineering, Nigde University Faculty of Engineering.
Plast Reconstr Surg. 2014 Apr;133(4):499e-510e. doi: 10.1097/PRS.0000000000000056.
This study investigated whether the in vivo osteogenic differentiation potential of adipose-derived mesenchymal stem cells is enhanced by 17β-estradiol.
Thirty Sprague-Dawley rats were randomized and divided into five experimental groups. For the surgical procedure, biparietal full-thickness bone defects (7 mm in diameter) were created. A chitosan-hydroxyapatite scaffold was used as the vehicle system for 17β-estradiol-loaded nanoparticles and adipose-derived mesenchymal stem cells. The first group, the blank defect group, was the control group. The defects were filled with either scaffold, estradiol, and scaffold; scaffold and adipose-derived mesenchymal stem cells; or estradiol, scaffold, and adipose-derived mesenchymal stem cells as experimental groups. The rats were killed at the end of weeks 4 and 12, and their calvariae were harvested for histologic and microtomographic evaluation.
Micro-computed tomographic evaluation of estradiol, scaffold, and adipose-derived mesenchymal stem cells revealed the highest median value (82.59 ± 17.17), and the difference was significant compared with the blank defect group (p = 0.004). Histologic samples demonstrated a significant difference between experimental groups for bone defect repair at the end of weeks 4 and 12 (p = 0.003 and p < 0.001). The estradiol, scaffold, and adipose-derived mesenchymal stem cell group had the highest median score (3.00 ± 0.0) at week 12, which was significantly higher than scores for the scaffold and adipose-derived mesenchymal stem cell group and the blank defect group.
17β-Estradiol appears to be a novel and promising agent for future cell-based bone tissue-engineering studies.
本研究旨在探讨 17β-雌二醇是否能增强脂肪间充质干细胞的体内成骨分化潜能。
30 只 Sprague-Dawley 大鼠随机分为 5 个实验组。对于手术过程，创建双额全层骨缺损（直径 7mm）。壳聚糖-羟基磷灰石支架作为载体制备 17β-雌二醇负载纳米颗粒和脂肪间充质干细胞。第一组，空白缺损组为对照组。缺损处分别填充支架、雌二醇、支架；支架和脂肪间充质干细胞；或雌二醇、支架和脂肪间充质干细胞作为实验组。在第 4 周和第 12 周末处死大鼠，取颅骨进行组织学和显微 CT 评价。
雌二醇、支架和脂肪间充质干细胞的显微 CT 评价显示中位数最高（82.59 ± 17.17），与空白缺损组差异有统计学意义（p = 0.004）。组织学样本显示，在第 4 周末和第 12 周末的骨缺损修复方面，实验组之间存在显著差异（p = 0.003 和 p < 0.001）。在第 12 周末，雌二醇、支架和脂肪间充质干细胞组的中位数评分为 3.00 ± 0.0，明显高于支架和脂肪间充质干细胞组和空白缺损组。
17β-雌二醇似乎是未来基于细胞的骨组织工程研究的一种新型有前途的药物。
