Department of Hematology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
Acta Biochim Biophys Sin (Shanghai). 2014 May;46(5):420-5. doi: 10.1093/abbs/gmu015. Epub 2014 Mar 27.
The pathological changes of erythrocytes are detected at the nanometer scale, which is important for revealing the onset of diseases and diagnosis. The aim of this study is to examine the ultrastructural changes of erythrocytes in Waldenström macroglobulinemia (WM) at a nanometer scale. Blood samples were collected from two healthy volunteers, two WM patients, and three multiple myeloma (MM) patients when they were first diagnosed. The changes of morphology in the erythrocytes were studied at the nanometer level by high-resolution atomic force microscopy imaging (AFM). Compared with the healthy controls and the MM patients, there were dramatic deformations in the overall shape and surface membrane of the erythrocytes in WM patients. Healthy, pathological WM, and MM erythrocytes could be distinguished by several morphological parameters, including the width, length, length-to-width ratio, valley, peak, peak-to-valley, and Ra. AFM is able to detect the morphological differences in the red blood cells from WM patients, healthy controls, and MM patients. Therefore, the erythrocyte morphology is an important parameter for the diagnosis of WM, which can be used to distinguish WM from MM. The changes of ultrastructure in red blood cells may provide a clue to reveal the mechanism of WM.
在纳米尺度上检测到红细胞的病理变化，这对于揭示疾病的发生和诊断非常重要。本研究旨在在纳米尺度上研究瓦尔登斯特伦巨球蛋白血症（WM）中红细胞的超微结构变化。采集了两名健康志愿者、两名 WM 患者和三名多发性骨髓瘤（MM）患者在首次诊断时的血液样本。通过高分辨率原子力显微镜成像（AFM）在纳米水平上研究红细胞的形态变化。与健康对照组和 MM 患者相比，WM 患者的红细胞整体形状和表面膜出现明显变形。通过几个形态学参数，包括宽度、长度、长宽比、谷、峰、峰谷和 Ra，可区分健康、病理性 WM 和 MM 红细胞。AFM 能够检测出 WM 患者、健康对照组和 MM 患者的红细胞形态差异。因此，红细胞形态是 WM 诊断的一个重要参数，可用于区分 WM 和 MM。红细胞超微结构的变化可能为揭示 WM 机制提供线索。
