Córdova-Guerrero Jesús, Hernández-Guevara Esteban, Ramírez-Zatarain Sandy, Núñez-Bautista Marco, Ochoa-Terán Adrián, Muñiz-Salazar Raquel, Montes-Ávila Julio, López-Angulo Gabriela, Paniagua-Michel Armando, Torres Gustavo A Nuño
Escuela de Ciencias de la Salud, Universidad Autónoma de Baja California, Ensenada, Baja California 22890, Mexico.
Centro de Graduados e Investigación en Química, Instituto Tecnológico de Tijuana, Tijuana, Baja California 22000, Mexico.
Int J Mol Sci. 2014 Mar 26;15(4):5277-91. doi: 10.3390/ijms15045277.
Staphylococcus aureus is one of the most common causes of nosocomial infections. The purpose of this study was the synthesis and in vitro evaluation of antimicrobial activity of 10 new 3-oxazolidin-2-one analogues on 12 methicillin resistant S. aureus (MRSA) clinical isolates. S. aureus confirmation was achieved via catalase and coagulase test. Molecular characterization of MRSA was performed by amplification of the mecA gene. Antimicrobial susceptibility was evaluated via the Kirby-Bauer disc diffusion susceptibility test protocol, using commonly applied antibiotics and the oxazolidinone analogues. Only (R)-5-((S)-1-dibenzylaminoethyl)-1,3-oxazolidin-2-one (7a) exhibited antibacterial activity at 6.6 μg. These results, allow us to infer that molecules such as 7a can be potentially used to treat infections caused by MRSA strains.
金黄色葡萄球菌是医院感染最常见的病因之一。本研究的目的是合成10种新的3-恶唑烷-2-酮类似物，并对其针对12株耐甲氧西林金黄色葡萄球菌（MRSA）临床分离株的抗菌活性进行体外评估。通过过氧化氢酶和凝固酶试验确认金黄色葡萄球菌。通过扩增mecA基因对MRSA进行分子特征分析。使用常用抗生素和恶唑烷酮类似物，通过 Kirby-Bauer 纸片扩散药敏试验方案评估抗菌药敏性。只有(R)-5-((S)-1-二苄基氨基乙基)-1,3-恶唑烷-2-酮(7a)在6.6μg时表现出抗菌活性。这些结果使我们推断，诸如化合物7a这样的分子可能可用于治疗由MRSA菌株引起的感染。
