Bing Zhu, Jian-ru Yang, Yao-quan Jiang, Shi-feng Chen
Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Chongqing Medical University, Linjiang Road 74, Yuzhong District, Chongqing, 400010, China,
Cell Biochem Biophys. 2014 Sep;70(1):327-31. doi: 10.1007/s12013-014-9916-5.
The objective of the study was to investigate angiogenesis in non-small lung cancer by measuring the expression of CD34. Immunohistochemistry was used to detect CD34 at the endothelial cell surface in 81 surgically resected non-small cell lung cancer specimens. CD34 immunohistochemistry had high specificity and sensitivity with minimal background, which enabled efficient identification of CD34-positive staining. Statistical analysis showed that increased microvessel density (MVD) was closely correlated to tumor progression as reflected by the clinical stage (P < 0.05). However, MVD was not significantly associated with lymph node metastasis and other clinical and pathological features (P > 0.05). In conclusion, microvascular density may play an important role in the development and progression of lung cancer.
本研究的目的是通过测量CD34的表达来调查非小细胞肺癌中的血管生成。采用免疫组织化学方法检测81例手术切除的非小细胞肺癌标本内皮细胞表面的CD34。CD34免疫组织化学具有高特异性和敏感性，背景极小，能够有效识别CD34阳性染色。统计分析表明，微血管密度（MVD）增加与临床分期所反映的肿瘤进展密切相关（P < 0.05）。然而，MVD与淋巴结转移及其他临床和病理特征无显著相关性（P > 0.05）。总之，微血管密度可能在肺癌的发生和发展中起重要作用。
