Laboratoire d'Immunogénétique Moléculaire (LIMT), EA 3034, Faculté de Médecine Purpan, Toulouse 3 (Université Paul Sabatier), Laboratoire d'immunologie, CHU de Toulouse, 1 avenue Jean Poulhes, TSA 50032, 31059, Toulouse cedex 9, France.
Immunogenetics. 2014 Jun;66(6):353-60. doi: 10.1007/s00251-014-0770-9. Epub 2014 Mar 28.
African green monkeys (AGM) are among the most widely used nonhuman primate models used in various fields of medical research. One species of AGM that originated from West Africa, Chlorocebus sabaeus, was introduced three centuries ago in the Caribbean islands. We present here a systematic study of the major histocompatibility complex (MHC) polymorphism of Caribbean AGM which is currently frequently used as an animal model. We studied 54 animals originated from Barbados (N=25) or Saint Kitts (N=29). The MHC polymorphism was characterized by means of 17 MHC microsatellites spread across MHC and DRB genotyping by DGGE sequencing. We defined nine frequent MHC haplotypes of which two were found in the two insular populations suggesting either past exchanges between the two populations or a common origin of the founders of the two populations. By the analysis of a previously described EST library, we characterized 38 MHC cDNA sequences (17 class I and 21 class II). In conclusion, we characterized for the first time the MHC polymorphism of Barbados and Saint Kitts AGM. We found a restricted polymorphism due to a founding effect, which is responsible for a strong bottleneck. The poorness of MHC polymorphism observed in the Caribbean AGM populations is similar to that observed in the Mauritian cynomolgus macaque population.
非洲绿猴（AGM）是医学研究各个领域中使用最广泛的非人类灵长类动物模型之一。原产于西非的绿猴亚种，Chlorocebus sabaeus，三百年前被引入加勒比海岛屿。我们在这里对目前经常用作动物模型的加勒比 AGM 的主要组织相容性复合体（MHC）多态性进行了系统研究。我们研究了 54 只来自巴巴多斯（N=25）或圣基茨（N=29）的动物。通过 DGGE 测序对 MHC 和 DRB 基因分型进行了 17 个 MHC 微卫星的 MHC 多态性特征分析。我们定义了九个常见的 MHC 单倍型，其中两个在两个岛屿群体中发现，这表明两个群体之间存在过去的交流或两个群体的创始者有共同的起源。通过对先前描述的 EST 文库的分析，我们鉴定了 38 个 MHC cDNA 序列（17 个 I 类和 21 个 II 类）。总之，我们首次对巴巴多斯和圣基茨 AGM 的 MHC 多态性进行了描述。我们发现由于创始效应导致多态性受限，这导致了强烈的瓶颈效应。在加勒比 AGM 群体中观察到的 MHC 多态性的匮乏与在毛里求斯猕猴群体中观察到的情况相似。
