Potocka-Bakłażec Małgorzata, Sakowicz-Burkiewicz Monika, Kuczkowski Jerzy, Pawełczyk Tadeusz, Stankiewicz Czesław, Sierszeń Wojciech, Jankowski Zbigniew, Buczny Jacek
Department of Otolaryngology, Medical University of Gdańsk, Smoluchowskiego17, 80-214, Gdańsk, Poland,
Eur Arch Otorhinolaryngol. 2015 Aug;272(8):1907-12. doi: 10.1007/s00405-014-3008-4. Epub 2014 Mar 28.
Persistent measles virus infections play a crucial role in the pathomechanism of otosclerosis. The study was undertaken to investigate the role of tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β) and osteoprotegerin (OPG) in otosclerotic bone remodeling and to assess the relation of TNF-α, OPG and IL-1β expression levels in otosclerotic stape footplates to the occurrence of measles virus infection. 61 patients with otosclerosis were treated surgically. Thirty-one stapes obtained from cadavers of people, who had died from a sudden cause were used as a control group. The presence of measles virus RNA and the expression levels of TNF-α, IL-1β and OPG in otosclerotic foci were assessed using one-step RT-PCR. The presence of measles virus RNA was noted in 80.3 % of otosclerotic stapes (49 out of 61) and 9.7 % of normal tissues (3 out of 31). Transcript of TNF-α, IL-1β and OPG was detected in 40, 46 and 18 virus-positive stapes, respectively. The transcript level of TNF-α and IL-1β was significantly higher in otosclerotic tissues comparing to normal tissue. The OPG expression level was significantly lower in otosclerotic tissues comparing to controls. The presence of measles virus RNA in the stapes may indicate its role in the pathogenesis of otosclerosis. The presence of TNF-α and IL-1β mRNA in the virus-positive stapes could be the result of viral antigen stimulation and may be a marker of inflammation the otosclerotic focus. The lack of OPG mRNA and the presence of TNF-α and IL-1β mRNA in the majority of otosclerotic tissues reflect the bone remodeling process occurring in the stapes.
持续性麻疹病毒感染在耳硬化症的发病机制中起关键作用。本研究旨在探讨肿瘤坏死因子-α（TNF-α）、白细胞介素1β（IL-1β）和骨保护素（OPG）在耳硬化性骨重塑中的作用，并评估耳硬化镫骨足板中TNF-α、OPG和IL-1β表达水平与麻疹病毒感染发生之间的关系。61例耳硬化症患者接受了手术治疗。从猝死人员尸体上获取的31个镫骨用作对照组。采用一步法RT-PCR评估耳硬化病灶中麻疹病毒RNA的存在以及TNF-α、IL-1β和OPG的表达水平。在80.3%的耳硬化镫骨（61例中的49例）和9.7%的正常组织（31例中的3例）中检测到麻疹病毒RNA。分别在40个、46个和18个病毒阳性镫骨中检测到TNF-α、IL-1β和OPG的转录本。与正常组织相比，耳硬化组织中TNF-α和IL-1β的转录水平显著更高。与对照组相比，耳硬化组织中OPG的表达水平显著更低。镫骨中麻疹病毒RNA的存在可能表明其在耳硬化症发病机制中的作用。病毒阳性镫骨中TNF-α和IL-1β mRNA的存在可能是病毒抗原刺激的结果，并且可能是耳硬化病灶炎症的标志物。大多数耳硬化组织中OPG mRNA的缺乏以及TNF-α和IL-1β mRNA的存在反映了镫骨中发生的骨重塑过程。
