Shigenaga Y, Nishimura M, Suemune S, Nishimori T, Doe K, Tsuru H
Department of Oral Anatomy (1st Division), Hiroshima University School of Dentistry, Japan.
Brain Res. 1989 Jan 16;477(1-2):66-89. doi: 10.1016/0006-8993(89)91395-4.
Transganglionic transport of horseradish peroxidase-wheat germ agglutinin conjugate (HRP-WGA) was used to study the somatotopic organization of pulpal afferent neurons innervating the different types of teeth in the trigeminal ganglion and trigeminal sensory nuclear complex (TSNC). In separate animals, the upper first 3 incisors (UI1-3), canine (UC), second premolar (UP2) and third premolar (UP3), and the lower first three incisors (LI1-3), canine (LC), first premolar (LP1), second premolar (LP2) and molar (LM) were traced in this experiment. Cell bodies innervating posterior teeth were found with greater frequency in dorsal maxillary ganglion regions, while somata supplying more anterior teeth were predominant ventrally. In contrast, cell bodies innervating the lower teeth were not arranged in a somatotopic fashion in the mandibular subdivision. Each pulpal afferent from lower and upper teeth projected to the subnucleus dorsalis (Vpd) of the pars principalis, the rostrodorsomedial (Vo.r) and dorsomedial parts (Vo.dm) of the pars oralis (Vo), the medial regions of the pars interpolaris (Vi), and laminae I, II, and V of the medullary dorsal horn, and terminal fields between the upper and lower teeth were separated in each subdivision. Pulpal projections from both the upper and lower teeth to each subdivision were organized in a somatotopic manner, while an extensive overlap in projections was noted between the adjoining teeth. In the Vpd, the upper and lower teeth were represented dorsoventrally, and projections from the anterior to posterior teeth in the upper jaw were arranged in both rostrocaudal and ventrodorsal sequences whereas those in the lower jaw were organized caudarostrally and lateromedially. In the Vo.r and Vo.dm, the upper and lower teeth were represented in a mediolateral sequence and projections from the anterior to posterior teeth were organized in a ventrolateral to dorsomedial sequence. In the Vi, pulpal projections were organized in a topographic fashion similar to that observed in the Vo.r and Vo.dm. In the medullary dorsal horn, the upper and lower teeth were represented in laminae I, II and V in a lateromedial sequence. Their projections to laminae I and V were topographically organized in a mediolateral and rostrocaudal sequence.(ABSTRACT TRUNCATED AT 400 WORDS)
采用辣根过氧化物酶-小麦胚凝集素复合物（HRP-WGA）的跨节运输来研究支配三叉神经节和三叉神经感觉核复合体（TSNC）中不同类型牙齿的牙髓传入神经元的躯体定位组织。在不同的动物中，本实验追踪了上颌的上前三颗切牙（UI1-3）、尖牙（UC）、第二前磨牙（UP2）和第三前磨牙（UP3），以及下颌的下前三颗切牙（LI1-3）、尖牙（LC）、第一前磨牙（LP1）、第二前磨牙（LP2）和磨牙（LM）。支配后牙的细胞体在上颌神经节背侧区域出现的频率更高，而支配更靠前牙齿的细胞体主要位于腹侧。相比之下，支配下颌牙齿的细胞体在下颌神经节分支中未呈躯体定位排列。来自上下颌牙齿的每一个牙髓传入纤维都投射到主部的背侧亚核（Vpd）、口部（Vo）的吻背内侧（Vo.r）和背内侧部分（Vo.dm）、极间部（Vi）的内侧区域以及延髓背角的I、II和V层，并且上下颌牙齿之间的终末场在每个分支中都是分开的。来自上下颌牙齿到每个分支的牙髓投射都以躯体定位的方式组织，而相邻牙齿之间的投射存在广泛重叠。在Vpd中，上下颌牙齿在背腹方向上呈现，上颌中从前牙到后牙的投射在前后和背腹序列中排列，而下颌中的投射则按尾头和内外侧方向组织。在Vo.r和Vo.dm中，上下颌牙齿按内外侧序列呈现，从前牙到后牙的投射按腹外侧到背内侧序列组织。在Vi中，牙髓投射以类似于在Vo.r和Vo.dm中观察到的地形方式组织。在延髓背角中，上下颌牙齿在I、II和V层中按内外侧序列呈现。它们向I层和V层的投射在内外侧和前后序列中按地形组织。（摘要截断于400字）
