Li Rachel W, Kirkland Nicholas T, Truong John, Wang Jian, Smith Paul N, Birbilis Nick, Nisbet David R
The Medical School, The Australian National University, Acton, ACT 0200, Australia; John Curtin School of Medical Research, The Australian National University, Acton, ACT 0200, Australia.
J Biomed Mater Res A. 2014 Dec;102(12):4346-57. doi: 10.1002/jbm.a.35111. Epub 2014 Feb 24.
The postdegradation effect of pure Mg, Mg-1Y, Mg-5Al, and Mg-2Ca alloys on the differentiation, proliferation and gene expression of human mesenchymal stem cells (hMSCs) was investigated. It was revealed that that Mg(2+) ions result in an increase in cell proliferation. However, we observed a maximum concentration (approximately 8.0 × 10(-4) M) that was favourable to ATP production, above which ATP production began to decrease. In contrast to proliferation, no maximum concentration for osteogenic differentiation was observed, with increasing concentration of Mg(2+) ions resulting in an increase in osteogenic differentiation across the entire tested range. Interestingly, the Mg-2Ca alloy had minimal effect on osteogenic differentiation, with Mg-1Y and pure Mg having a superior effect on the proliferation and differentiation of hMSCs. This was also observed from gene expression data, where these alloys upregulated TGFβ-1, SMAD4, FGF-2, FGF-10, and BMP-2, while SOX-2, SOX-9, and TNF-α were downregulated. Increased expression of TGFβ-1, SMAD4, BMPs, and COLIA1 protein provided further evidence to support osteogenic differentiation and that the influence of the alloying extracts on differentiation may be via the SMAD signaling pathway.
研究了纯镁、Mg-1Y、Mg-5Al和Mg-2Ca合金降解后对人间充质干细胞（hMSCs）分化、增殖和基因表达的影响。结果表明，Mg(2+)离子可导致细胞增殖增加。然而，我们观察到有利于ATP产生的最大浓度（约8.0×10(-4) M），超过该浓度后ATP产生开始下降。与增殖情况相反，未观察到成骨分化的最大浓度，随着Mg(2+)离子浓度的增加，在整个测试范围内成骨分化均增加。有趣的是，Mg-2Ca合金对成骨分化的影响最小，Mg-1Y和纯镁对hMSCs的增殖和分化具有更好的作用。从基因表达数据中也观察到了这一点，这些合金上调了TGFβ-1、SMAD4、FGF-2、FGF-10和BMP-2，而下调了SOX-2、SOX-9和TNF-α。TGFβ-1、SMAD4、骨形态发生蛋白（BMPs）和COLIA1蛋白表达的增加为支持成骨分化提供了进一步的证据，并且合金提取物对分化的影响可能是通过SMAD信号通路实现的。
