Feng Bei, Duan Huichuan, Fu Wei, Cao Yilin, Jie Zhang Wen, Zhang Yanzhong
College of Chemistry, Chemical Engineering, and Biotechnology, and State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai, 201620, China; Department of Cardiothoracic Surgery, Institute of Pediatric Translational Medicine, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, 1678 Dongfang Road, Shanghai, 200127, China.
J Biomed Mater Res A. 2015 Feb;103(2):431-8. doi: 10.1002/jbm.a.35184. Epub 2014 Apr 9.
In this article, gelatin (GT) and polycaprolactone (PCL) blended with a weight ratio of 50:50 were dissolved in the trifluoroethanol (TFE) or the acetic acid-doped TFE solvent system (0.2% relative to TFE) to prepare fibrous scaffolds of GT/PCL with different compositional and morphological homogeneities (denoted as the group 1 and the group 2 scaffolds) by electrospinning. The morphology and composition of the two groups of fibrous scaffolds were examined by scanning electron microscopy and Fourier transform infrared spectroscopy, respectively. Then, using green fluorescence protein-labeled mouse fibroblasts and HaCaT cells (a human keratinocyte cell line) as the model cells, cell adhesion, morphology, and proliferation were assessed by laser scanning confocal microscopy, scanning electron microscopy, and cell counting kit-8 assay, respectively. The results showed that the morphological and compositional inhomogeneity of the group 1 scaffolds had a remarkable influence on cell adhesion and proliferation. In contrast, there was no significant difference among the group 2 scaffolds because of their good consistency in fiber morphology and composition. Phase separation resultant GT content variance in the group 1 scaffolds is suggested as one of the major causes. This study highlighted the importance of producing morphologically uniform and compositionally homogeneous composite nanofibers while electrospinning natural and synthetic polymer blends.
在本文中，将重量比为50:50的明胶（GT）和聚己内酯（PCL）溶解于三氟乙醇（TFE）或乙酸掺杂的TFE溶剂体系（相对于TFE为0.2%）中，通过静电纺丝制备具有不同组成和形态均匀性的GT/PCL纤维支架（分别记为第1组和第2组支架）。分别通过扫描电子显微镜和傅里叶变换红外光谱对两组纤维支架的形态和组成进行了检测。然后，以绿色荧光蛋白标记的小鼠成纤维细胞和HaCaT细胞（一种人角质形成细胞系）作为模型细胞，分别通过激光扫描共聚焦显微镜、扫描电子显微镜和细胞计数试剂盒-8检测评估细胞黏附、形态和增殖情况。结果表明，第1组支架的形态和组成不均匀性对细胞黏附和增殖有显著影响。相比之下，第2组支架由于其纤维形态和组成具有良好的一致性，差异不显著。第1组支架中相分离导致的GT含量变化被认为是主要原因之一。本研究强调了在静电纺丝天然和合成聚合物共混物时制备形态均匀且组成均一的复合纳米纤维的重要性。
