Department of Stomatology, General Hospital of the Air Force of Chinese PLA, Beijing, 100853, China.
Cell Biol Int. 2014 Jun;38(6):729-36. doi: 10.1002/cbin.10257. Epub 2014 Feb 20.
Estrogen deficiency-induced osteoporosis typically occurs in postmenopausal women and has been strongly associated with periodontal diseases. Periodontal ligament stem cells (PDLSCs) isolated from the periodontal ligament can differentiate into many types of specialized cells, including osteoblast-like cells that contribute to periodontal tissue repair. The Notch signaling pathway is highly conserved and associated with self-renewal potential and cell-fate determination. Recently, several studies have focused on the relationship between Notch signaling and osteogenic differentiation. However, the precise mechanisms underlying this relationship are largely unknown. We have successfully isolated PDLSCs from both ovariectomized (OVX) and sham-operated rats. Both the mRNA and protein levels of Notch1 and Jagged1 were upregulated when PDLSCs were cultured in osteogenic induction media. Mineralization assays showed decreased calcium deposits in OVX-PDLSCs treated with a γ-secretase inhibitor compared with control cells. Thus Notch signaling is important in maintaining the osteogenic differentiation of PDLSCs in osteoporotic rats, which help in the development of a potential therapeutic strategy for periodontal disease in postmenopausal women.
雌激素缺乏诱导的骨质疏松症通常发生在绝经后妇女中，并且与牙周病密切相关。从牙周韧带中分离出来的牙周韧带干细胞（PDLSCs）可以分化为多种类型的特化细胞，包括成骨样细胞，这些细胞有助于牙周组织修复。Notch 信号通路高度保守，与自我更新潜能和细胞命运决定有关。最近，有几项研究集中在 Notch 信号与成骨分化之间的关系上。然而，这种关系的确切机制在很大程度上尚不清楚。我们已经成功地从去卵巢（OVX）和假手术大鼠中分离出 PDLSCs。当 PDLSCs 在成骨诱导培养基中培养时，Notch1 和 Jagged1 的 mRNA 和蛋白水平均上调。矿化试验显示，与对照细胞相比，用 γ-分泌酶抑制剂处理的 OVX-PDLSCs 中的钙沉积减少。因此，Notch 信号在维持骨质疏松大鼠 PDLSCs 的成骨分化中起重要作用，这有助于开发绝经后妇女牙周病的潜在治疗策略。
