Park Honghyun, Lee Kuen Yong
Department of Bioengineering, Hanyang University, Seoul, 133-791, Republic of Korea.
J Biomed Mater Res A. 2014 Dec;102(12):4519-25. doi: 10.1002/jbm.a.35126. Epub 2014 Feb 26.
Despite the widespread use of alginate (AL) hydrogels in many biomedical applications, including tissue engineering, AL is inherently non-degradable under physiological conditions. We hypothesized that degradable alginate (dAL) would be useful for cartilage regeneration when combined with hyaluronate (HA). We prepared dAL by partial oxidation of AL using sodium periodate, and the degradation rate of AL hydrogel was able to be regulated by control of sodium periodate concentration. The degradable oxidized AL/HA gels were formed in the presence of cells and their characteristics were investigated. dAL/HA gels with primary chondrocytes were injected subcutaneously into mice. Effective cartilage regeneration was observed after 6 weeks of transplantation based on histological analysis. Moreover, substantial secretion of sulfated glycosaminoglycans and expression of chondrogenic marker genes were also observed compared with non-dAL/HA gels. These results indicate that dAL/HA hydrogels may be useful in cartilage regeneration, and in many tissue engineering applications.
尽管藻酸盐（AL）水凝胶在包括组织工程在内的许多生物医学应用中被广泛使用，但在生理条件下，AL本质上是不可降解的。我们假设，可降解藻酸盐（dAL）与透明质酸盐（HA）结合时对软骨再生有用。我们使用高碘酸钠通过对AL进行部分氧化来制备dAL，并且通过控制高碘酸钠的浓度能够调节AL水凝胶的降解速率。在细胞存在的情况下形成了可降解的氧化AL/HA凝胶，并对其特性进行了研究。将含有原代软骨细胞的dAL/HA凝胶皮下注射到小鼠体内。基于组织学分析，在移植6周后观察到有效的软骨再生。此外，与非dAL/HA凝胶相比，还观察到硫酸化糖胺聚糖的大量分泌和成软骨标记基因的表达。这些结果表明，dAL/HA水凝胶可能在软骨再生以及许多组织工程应用中有用。
