Niyonzima Francois Niyongabo, More Sunil
a Department of Biochemistry, Center for Post Graduate Studies , Jain University , Bangalore , India.
Prep Biochem Biotechnol. 2015;45(3):233-58. doi: 10.1080/10826068.2014.907183.
Proteases are one of the most important commercial enzymes used in various industrial domains such as detergent and leather industries. The alkaline proteases as well as other detergent-compatible enzymes such as lipases and amylases serve now as the key components in detergent formulations. They break down various stains during fabric washing. The search for detergent-compatible proteases with better properties is a continuous exercise. The current trend is to use detergent-compatible proteases that are stable over a wide temperature range. Although the proteases showing stability at elevated pH have the capacity to be used in detergent formulations, their usage can be significant if they are also stable and compatible with detergent and detergent ingredients, and also able to remove protein stains. Despite the existence of some reviews on alkaline proteases, there is no specification for the use of alkaline proteases as detergent additives. The present review describes the detergent-compatible proteases tested as detergent additives. An overview was provided for screening, optimization, purification, and properties of detergent compatible proteases, with an emphasis on the stability and compatibility of the alkaline proteases with the detergent and detergent compounds, as well as stain removal examination methods.
蛋白酶是洗涤剂和皮革工业等各种工业领域中使用的最重要的商业酶之一。碱性蛋白酶以及其他与洗涤剂兼容的酶，如脂肪酶和淀粉酶，现在是洗涤剂配方中的关键成分。它们在织物洗涤过程中分解各种污渍。寻找具有更好性能的与洗涤剂兼容的蛋白酶是一项持续的工作。当前的趋势是使用在很宽温度范围内都稳定的与洗涤剂兼容的蛋白酶。尽管在较高pH值下显示出稳定性的蛋白酶有能力用于洗涤剂配方中，但如果它们还稳定且与洗涤剂和洗涤剂成分兼容，并且能够去除蛋白质污渍，那么它们的用途可能会很广泛。尽管已经有一些关于碱性蛋白酶的综述，但对于将碱性蛋白酶用作洗涤剂添加剂并没有具体说明。本综述描述了作为洗涤剂添加剂测试的与洗涤剂兼容的蛋白酶。提供了关于与洗涤剂兼容的蛋白酶的筛选、优化、纯化和性质的概述，重点是碱性蛋白酶与洗涤剂和洗涤剂化合物的稳定性和兼容性，以及去污效果检测方法。
