Cikman Oztekin, Ozkan Adile, Aras Adem Bozkurt, Soylemez Omer, Alkis Hilal, Taysi Seyithan, Karaayvaz Muammer
1Department of General Surgery, Faculty of Medicine, Canakkale 18 March University, Canakkale, Turkey.
J Invest Surg. 2014 Oct;27(5):262-6. doi: 10.3109/08941939.2014.898811. Epub 2014 Mar 28.
Many cancer patients treated with radiotherapy suffer severe side effects during and after their treatment. The aim of this study was to investigate the effects of irradiation and the addition of Nigella sativa oil (NSO) on the oxidant/antioxidant system in the liver tissue of irradiated rats.
A total of 24 Sprague-Dawley rats were randomly distributed into three groups of equal numbers. The control group received neither NSO nor irradiation but received 1-ml saline orally. The irradiation group (IR) received total head 5 gray (Gy) of gamma irradiation as a single dose, plus 1-ml saline orally. The IR plus NSO group received both total head 5 Gy of gamma irradiation as a single dose and 1 g/kg/day NSO orally through an orogastric tube starting one hour before irradiation and continuing for 10 days.
While liver tissue total oxidant status (TOS), lipid hydroperoxide (LOOH) level, and oxidative stress index (OSI) were significantly increased in the IR group compared to the control group, total antioxidant status (TAS), sulfhydryl (-SH) levels, and PON activity were significantly decreased. Cp activity in the IR plus NSO and IR groups was higher than in the control group. ARYL activity in the IR plus NSO supplemented group was higher than that in other groups.
NSO reduces oxidative stress markers and has antioxidant effects, which also augments the antioxidant capacity in the liver tissue of rats.
许多接受放射治疗的癌症患者在治疗期间及之后会遭受严重的副作用。本研究的目的是调查辐射以及添加黑种草籽油（NSO）对受辐射大鼠肝脏组织中氧化/抗氧化系统的影响。
总共24只Sprague-Dawley大鼠被随机分成数量相等的三组。对照组既不接受NSO也不接受辐射，而是口服1毫升生理盐水。辐射组（IR）接受单次头部5格雷（Gy）的γ射线辐射，外加口服1毫升生理盐水。IR加NSO组接受单次头部5 Gy的γ射线辐射，并且在辐射前1小时开始通过胃管口服1克/千克/天的NSO，并持续10天。
与对照组相比，IR组肝脏组织的总氧化剂状态（TOS）、脂质过氧化氢（LOOH）水平和氧化应激指数（OSI）显著升高，而总抗氧化剂状态（TAS）、巯基（-SH）水平和对氧磷酶（PON）活性显著降低。IR加NSO组和IR组的铜蓝蛋白（Cp）活性高于对照组。补充NSO的IR加NSO组的芳基酯酶（ARYL）活性高于其他组。
NSO可降低氧化应激标志物并具有抗氧化作用，这也增强了大鼠肝脏组织中的抗氧化能力。
