Üstün K, Taysı S, Sezer U, Demir E, Baysal E, Demir T, Sarıçiçek E, Alkış H, Senyurt S Z, Tarakçıoğlu M, Aksoy N
Department of Periodontology, Faculty of Dentistry, Gaziantep University, Gaziantep, Turkey.
Oral Dis. 2014 Jan;20(1):109-13. doi: 10.1111/odi.12082. Epub 2013 Feb 28.
The aim of this study was to evaluate the effects of Nigella sativa (N.S.) oil against radiation-induced oxidative stress in the rat tongue.
Thirty-two Sprague-Dawley rats were randomly divided into four equal groups. Group 1 [control group(C)] did not receive N.S. oil or irradiation. Group 2 [sham control group(CN)] did not receive N.S. oil or irradiation but received 1-ml saline orally, plus sham irradiation. Group 3 [irradiation group(R)] received irradiation, plus 1-ml saline orally. Group 4 [irradiation plus N.S. oil group(RN)] received irradiation plus 1 g kg(-1) per day of N.S. oil orally for 10 days. The animals were euthanized on day 10, and tongue tissues were collected for evaluating biochemical oxidative parameters.
The oxidative stress index, total oxidant status and lipid hydroperoxides levels in the R group were statistically higher than those in the C, CN and RN groups. The paraoxonase levels in the R group were statistically lower than those in the C, CN and RN groups. No statistically significant differences were detected between any of the groups, in terms of total antioxidant status and the arylesterase, ceruloplasmin and total sulfhydryl group levels.
Nigella sativa (N.S.) oil may be a beneficial agent in protecting against ionizing radiation-related tissue injury.
本研究旨在评估黑种草（N.S.）油对大鼠舌部辐射诱导的氧化应激的影响。
32只Sprague-Dawley大鼠随机分为四组，每组数量相等。第1组[对照组（C）]未接受黑种草油或辐射。第2组[假手术对照组（CN）]未接受黑种草油或辐射，但口服1毫升生理盐水，并接受假辐射。第3组[辐射组（R）]接受辐射，并口服1毫升生理盐水。第4组[辐射加黑种草油组（RN）]接受辐射，并口服每天1克/千克的黑种草油，持续10天。在第10天对动物实施安乐死，并收集舌组织以评估生化氧化参数。
R组的氧化应激指数、总氧化剂状态和脂质氢过氧化物水平在统计学上高于C、CN和RN组。R组的对氧磷酶水平在统计学上低于C、CN和RN组。在总抗氧化状态以及芳基酯酶、铜蓝蛋白和总巯基水平方面，各实验组均未检测到统计学上的显著差异。
黑种草（N.S.）油可能是预防电离辐射相关组织损伤的有益药物。
