Department of Dental Biomaterials, Nihon University School of Dentistry at Matsudo, 870-1 Sakaecho Nishi 2, Matsudo, Chiba 271-8587, Japan.
Department of Biochemistry, Nihon University School of Dentistry at Matsudo, 870-1 Sakaecho Nishi 2, Matsudo, Chiba 271-8587, Japan.
J Biosci Bioeng. 2014 Sep;118(3):333-40. doi: 10.1016/j.jbiosc.2014.02.016. Epub 2014 Mar 26.
We have recently identified plasma-irradiated silk fibroin (P-AF) as a key regulator of bone matrix properties and composition. Bone matrix properties were tested in 48 femur critical size defects (3.25 mm in diameter) with the expression of osteoblast specific genes at 1 and 2 weeks after surgery. The scaffolds were characterized by various states of techniques; the scanning electronic microcopy revealed the large sized pores in the aqueous-based silk fibroin (A-F) scaffold and showed no alteration into the architecture by the addition of plasma irradiation. The contact angle measurements confirmed the introduction of plasma helped to change the hydrophobic nature into hydrophilic. The histological analyses confirmed the presence of silk fibroin in scaffolds and newly formed bone around the scaffolds. Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-β, TGF-β type III receptor, Runx2, type I collagen and osteocalcin). These data were the first to show that the properties of bone matrix are regulated, specifically through Runx2 pathway in P-AF group. Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
我们最近发现，等离子体辐照丝素蛋白（P-AF）是调节骨基质特性和组成的关键调节剂。在手术后 1 周和 2 周时，通过检测成骨细胞特异性基因，测试了骨基质特性。该支架通过各种技术状态进行了表征；扫描电子显微镜显示，水基丝素蛋白（A-F）支架中存在大尺寸的孔，并且添加等离子体辐照后不会改变其结构。接触角测量证实，等离子体的引入有助于将疏水性改变为亲水性。组织学分析证实了支架中存在丝素蛋白以及支架周围新形成的骨。免疫组织化学检查显示了一组成骨细胞特异性基因（TGF-β、TGF-β III 型受体、Runx2、I 型胶原和骨钙素）的表达模式增加。这些数据首次表明，骨基质的特性受到调节，特别是在 P-AF 组中通过 Runx2 途径。因此，P-AF 的应用增加了骨的几种组成特性，包括增加骨基质、矿物质浓度、皮质厚度和小梁骨体积。
