Department of Pharmacology, Nihon University School of Dentistry, Matsudo, Chiba 271-8587, Japan.
J Biomed Mater Res B Appl Biomater. 2013 Feb;101(2):295-302. doi: 10.1002/jbm.b.32839. Epub 2012 Nov 2.
Porous scaffolds were prepared using regenerated Bombyx mori silk fibroin dissolved in water or hexafluoroisopropanol (HFIP). The effects of these two preparations on the formation and growth of new bone on implantation into the rabbit femoral epicondyle was examined. The aqueous-based fibroin (A-F) scaffold exhibited significantly greater osteoconductivity as judged by bone volume, bone mineral content, and bone mineral density at the implant site than the HFIP-based fibroin (HFIP-F) scaffold. Micro-CT analyses showed that the morphology of the newly formed bone differed significantly in the two types of silk fibroin scaffold. After 4 weeks of implantation, new trabecular bone was seen inside the pores of the A-F scaffold implant while the HFIP-F scaffold only contained necrotic cells. No trabecular bone was seen within the pores of the latter scaffolds, although the pores of these did contain giant cells and granulation tissue.
采用再生丝素蛋白制备多孔支架，该丝素蛋白溶解于水或六氟异丙醇（HFIP）中。研究了这两种方法对植入兔股骨髁后新骨形成和生长的影响。水基丝素（A-F）支架的骨体积、骨矿物质含量和骨矿物质密度显著大于 HFIP 基丝素（HFIP-F）支架，表现出更好的骨诱导性。Micro-CT 分析表明，两种丝素蛋白支架中新生骨的形态有显著差异。植入 4 周后，A-F 支架植入物的孔隙内可见新生小梁骨，而 HFIP-F 支架内仅含有坏死细胞。后者支架的孔隙内未见小梁骨，但这些支架的孔隙内含有巨细胞和肉芽组织。
