Department of Bioengineering, National University of Singapore, Singapore.
Biomaterials. 2013 Aug;34(24):5947-57. doi: 10.1016/j.biomaterials.2013.04.046. Epub 2013 May 13.
In this article, low crystallinity hydroxyapatite (LHA) is developed and utilized to modify silk fibroin scaffolds which are applied to repair bone/ligament defects successfully. It can promote osteogenesis which is authenticated through in vitro and in vivo tests. The scaffold is an efficient carrier, supporting cell proliferation and differentiation. Meanwhile, cytocompatibility and osteoblastic gene expressions (RUNX2 and osteocalcin, for example) of rabbit's bone marrow derived mesenchymal stem cells (MSCs) are significantly boosted on LHA/silk scaffold. Further, for animal trial, almost 60% of bone volume and 80% of original mechanical strength are recovered after 4 months' bone/ligament regeneration in bone tunnel of rabbit model, where significant amount of bone tissue regeneration is also confirmed by data of histological evaluation and micro computed tomography (μ-CT). Hence, the invented scaffold is applicable for ligament/bone regeneration in future lager animal and clinical trials.
本文开发并利用低结晶羟磷灰石（LHA）修饰丝素蛋白支架，成功应用于修复骨/韧带缺损。通过体外和体内实验证实其具有促进成骨作用。支架是一种有效的载体，支持细胞的增殖和分化。同时，LHA/丝素支架显著提高了兔骨髓间充质干细胞（MSCs）的细胞相容性和成骨基因表达（如 RUNX2 和骨钙素）。此外，在动物试验中，在兔模型的骨隧道中进行 4 个月的骨/韧带再生后，骨体积恢复了近 60%，原始机械强度恢复了 80%，组织学评估和微计算机断层扫描（μ-CT）的数据也证实了大量的骨组织再生。因此，该发明的支架适用于未来大型动物和临床试验中的韧带/骨再生。
